| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,176,010 | 0 | T | G | 25.4% | 126.8 / 12.0 | 55 | A37A (GCA→GCC) | fhuE | ferric‑rhodotorulic acid outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base T (6/35); new base G (14/0); total (20/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.90e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTG > REL606/1175937‑1176084 | cGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGgc > 1:1454638/1‑76 (MQ=255)agCTGTAATCATTTNCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTgc > 1:838559/2‑76 (MQ=255) gCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTgca < 1:755031/76‑1 (MQ=255) tGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGgcagc > 1:22416/1‑76 (MQ=255) gTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTgcagca < 2:241514/76‑1 (MQ=255) aTTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGGAGCAAAAGCg > 1:453596/1‑76 (MQ=255) aTTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCg > 1:82957/1‑76 (MQ=255) ttttCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGGAGCAAAAGCgg > 2:255786/1‑76 (MQ=255) ttCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGGGGCAAAAGCggga > 1:914596/1‑74 (MQ=255) tCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAg < 1:21208/76‑1 (MQ=255) gCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGCGGAGCAAAAGCGGCCGaa > 1:103870/1‑76 (MQ=255) ccATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCCGCAAAAAGGGCCGAAg > 2:19655/1‑76 (MQ=255) ccATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGGAAAAGCGGgaggag > 2:504169/1‑76 (MQ=255) ccATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAg > 1:926055/1‑76 (MQ=255) catcatCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCCGCCAAAGCGGCAGAAgg > 1:1416297/1‑76 (MQ=255) tcatcTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAggga > 1:6698/1‑74 (MQ=255) atcTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAAt < 2:144247/76‑1 (MQ=255) cTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCCAAAGCGGCAGGAGGGAATAg > 2:334455/1‑76 (MQ=255) tGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGGAGCAAAAGCGGCAGGAGGGAATAgg > 2:314248/1‑75 (MQ=255) ggATCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTg < 1:388084/76‑1 (MQ=255) ggAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGGAATAGTg > 1:575333/1‑76 (MQ=255) gCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCa < 1:38161/76‑1 (MQ=255) gCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCa < 1:236651/76‑1 (MQ=255) cTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAg < 1:712212/76‑1 (MQ=255) cTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCGGCAAAAGCGGCAGAAGGGAATAGTGcccg > 2:41760/1‑74 (MQ=255) tgtgGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGt < 1:397568/76‑1 (MQ=255) tgtgGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGGAATAGTGCCAGt > 2:1445830/1‑76 (MQ=255) gtgGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTg < 1:606979/76‑1 (MQ=255) gtgGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTg < 1:1409448/76‑1 (MQ=255) gtgGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTg < 1:841771/76‑1 (MQ=255) tgGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGc < 1:184379/76‑1 (MQ=255) ggCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGGAGCAAAAGCGGCAGGAGGGAATAGTGCCAGTGCt > 1:562180/1‑76 (MQ=255) gCTGAACCCTCAACAATCCCCTTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGGGCTa < 2:603183/76‑1 (MQ=255) gCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTa < 2:137678/76‑1 (MQ=255) gCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGGAATAGTGCCAGTGCTa > 1:63782/1‑76 (MQ=255) aaCCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGGAATAGTGCCAGTGCTATGCa > 2:607675/1‑76 (MQ=255) cccTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAAc < 2:523452/76‑1 (MQ=255) ccTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAAcc < 1:796391/76‑1 (MQ=255) ccTCAACAATCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAAcc > 2:90310/1‑76 (MQ=255) cTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCg < 2:1349090/76‑1 (MQ=255) caacaaTCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGGAATAGTGCCAGTGCTATGCAACCCGc > 2:267495/1‑76 (MQ=255) acaaTCACCGTTTCTTCAGTGGCTGGGGCAGCAAAAGCGGCAGGAGGTAATAGCGCCAGTGCTATGCAACCCGCaa > 1:642277/1‑76 (MQ=255) aaTCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAagt < 2:963672/76‑1 (MQ=255) aTCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAagta < 1:998358/76‑1 (MQ=255) aTCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAagta < 2:183607/76‑1 (MQ=255) aCCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTg < 1:17497/76‑1 (MQ=255) ccGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGa < 2:157566/76‑1 (MQ=255) ccGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGa < 2:878561/76‑1 (MQ=255) cGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGAc < 1:1478826/76‑1 (MQ=255) cttcAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGttt < 2:757100/76‑1 (MQ=255) cttcAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGGAATAGTGCCAGTGCTATGCAACCGGCAAGGAGGGACGGgtt > 2:441469/1‑76 (MQ=255) ttcAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTg < 2:115170/76‑1 (MQ=255) tcAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTgg < 2:313888/76‑1 (MQ=255) tcAGTGGCTGGGGCAGCAAAAGCGGCAGAAGGGAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGgtttg > 2:1065779/1‑74 (MQ=255) cAGTGGCTGGGGGAGCAAAAGCGGCAGAAGGGAATAGTGCCAGGGCTATGCAACCGGCATGTAGTGGCGGGTTggg > 2:357644/1‑75 (MQ=255) gTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGa < 2:1038034/76‑1 (MQ=255) tggctggcGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGAt > 1:980849/1‑76 (MQ=255) tGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGAt > 2:1504055/1‑76 (MQ=255) ggCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGTGGATg > 2:1520586/1‑76 (MQ=255) gCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATgg < 2:961497/76‑1 (MQ=255) gCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATgg < 1:441470/76‑1 (MQ=255) cTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGc > 1:108431/1‑76 (MQ=255) tGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCt > 2:736843/1‑76 (MQ=255) gggTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCt < 1:528841/75‑1 (MQ=255) ggTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCtt < 2:651330/76‑1 (MQ=255) ggTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCtt < 2:846065/76‑1 (MQ=255) ggTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCtt < 1:588881/76‑1 (MQ=255) ggTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCtt < 2:1457946/76‑1 (MQ=255) gTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTg < 1:95161/76‑1 (MQ=255) | CGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTG > REL606/1175937‑1176084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |