| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,393,061 | 0 | T | G | 44.4% | 41.3 / 15.7 | 36 | V211G (GTG→GGG) | aidB | isovaleryl CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (5/15); new base G (16/0); total (21/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.22e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCAGGGCGGTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTG > REL606/4392989‑4393134 | aGCAGGGCGGTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTggg < 2:773174/76‑1 (MQ=255) gCAGGGCGGTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTgggg < 2:302386/76‑1 (MQ=255) aGGGCGGTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑Ggggggggg > 1:360049/1‑74 (MQ=255) aGGGCGGTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCa < 1:1448364/76‑1 (MQ=255) cGGTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATaaa < 1:542068/76‑1 (MQ=255) ggTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGGGTAAAt > 2:622840/1‑76 (MQ=255) tCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGGTAAATGGt > 1:1385963/1‑76 (MQ=255) gATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGtttt < 2:1512107/76‑1 (MQ=255) gATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGGTAAATGGgttt > 1:174770/1‑76 (MQ=255) tGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGGGGGGGCGAAAAGGGttttt > 1:567650/1‑76 (MQ=255) tGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTtc < 1:1499662/76‑1 (MQ=255) gTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGATAAAAGGgtttttt > 1:265814/1‑74 (MQ=255) gTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGCATAAATGGttttttt > 1:3166/1‑74 (MQ=255) aTGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCgg < 1:306095/76‑1 (MQ=255) acacCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCa < 1:1448903/76‑1 (MQ=255) ccaccCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAg < 1:1444916/76‑1 (MQ=255) ccaccCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGCTAAATGGTTTTTTTTGGTTTCGCAAAg > 2:612869/1‑76 (MQ=255) accCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGGATAAATTGGTTTTTTCGGCTCCGCCAAGCg > 1:223644/1‑76 (MQ=255) accCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCg < 2:421349/76‑1 (MQ=255) cccGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGATAAATGGGTTTTTTCGGGTCCGCAAAGCGa > 2:494701/1‑76 (MQ=255) cccGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGCATAAATGGGTTTTCTCGGGTCCGCAAAGCGa > 1:927917/1‑76 (MQ=255) cGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATg < 2:1137419/76‑1 (MQ=255) cGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGCATAAATGGTTTTTTTCGGTTCCGCCAAGCGATg > 2:787392/1‑76 (MQ=255) gTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATgc > 1:1524252/1‑76 (MQ=255) cAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGATAAATGGTTTTTTTCGGTTCCGCAAAGCGATgcgcc > 2:924972/1‑75 (MQ=255) gCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTg < 2:782570/76‑1 (MQ=255) gTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGt < 1:161402/76‑1 (MQ=255) gTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGGGCATCTggg > 2:207407/1‑75 (MQ=255) gTCTGGAAGATGGCTCTTATCGGCT‑GGGGGGGGATAAATGGTTTTTCTCGGTTCCGCAAACCGAAGCGCATATggg > 2:900698/1‑75 (MQ=255) gAAGATGGCTCGTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTgg < 1:68444/76‑1 (MQ=255) aaGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGgc < 1:175599/76‑1 (MQ=255) aaGATGGCTCTTATCGGCT‑GGGGGGGCATAAATGGGTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGgc > 1:1088384/1‑76 (MQ=255) tGGCTCTTATCGGCT‑GGTGGGGCATAAAAGGTTTTTCTCGGTTCCCCGAAGCGGTGCGCGTCTGGTGCTGGGGCAg > 2:253298/1‑76 (MQ=255) ctctTATCGGCT‑GGGGGGGCATAAATGGTTTTTCTCGGTTCCGCCAAGCGATGCGCATCTGGGGCTGGCGCAGAcc > 2:340801/1‑76 (MQ=255) tctTATCGGCT‑GGGGGGGCATAAATGGTTTTTCTCAGTTCCGCAAAGCGATGCGCCTCTGGTGCTGGCGCCGACcg > 2:1368061/1‑76 (MQ=255) ggCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGggtgg > 1:41541/1‑76 (MQ=255) cT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTc > 2:1403089/1‑76 (MQ=255) t‑ggtggGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCt > 2:755969/1‑76 (MQ=255) t‑ggtggGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCt > 2:1277824/1‑76 (MQ=255) ggtggGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTg < 2:282931/76‑1 (MQ=255) | AGCAGGGCGGTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCT‑GGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTG > REL606/4392989‑4393134 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |