| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 433,653 | T→G | F50V (TTT→GTT) | hupB → | HU, DNA‑binding transcriptional regulator, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 433,653 | 0 | T | G | 95.6% | 184.0 / ‑1.3 | 45 | F50V (TTT→GTT) | hupB | HU, DNA‑binding transcriptional regulator, beta subunit |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (25/18); total (26/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCT > REL606/433582‑433724 | aTGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGc < 1:1434828/76‑1 (MQ=255) gCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCg < 2:58608/76‑1 (MQ=255) attattGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTa < 2:878571/76‑1 (MQ=255) ttattGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTaa < 1:444298/76‑1 (MQ=255) tattGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGCTaaa > 2:1017581/1‑76 (MQ=255) tGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAagag > 2:418414/1‑76 (MQ=255) gCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGc > 2:126093/1‑76 (MQ=255) gCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGc > 1:1024828/1‑76 (MQ=255) ccGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGtgc > 1:335545/1‑76 (MQ=255) ccGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGtgc < 1:639745/76‑1 (MQ=255) gTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGtgctg > 2:690502/1‑76 (MQ=255) gTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGtgctg > 2:726764/1‑76 (MQ=255) tAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGtgctgc > 2:701186/1‑76 (MQ=255) aaCTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGcc > 2:1436876/1‑76 (MQ=255) aCTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTATAGAGCGTGCTGcca < 1:846090/76‑2 (MQ=255) cTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCg < 2:276333/76‑1 (MQ=255) tGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGt < 2:397689/76‑1 (MQ=255) aaTCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGCGTGCTGCCCGTAc < 1:25712/76‑1 (MQ=255) tctcTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTg > 2:597322/1‑76 (MQ=255) gAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGc > 1:144736/1‑76 (MQ=255) gAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGc > 2:1425863/1‑76 (MQ=255) aaaGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCa > 2:983174/1‑76 (MQ=255) aaaGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCa < 2:1026904/76‑1 (MQ=255) aaaGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCa < 1:1403126/76‑1 (MQ=255) aagaagGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCaa > 2:1267365/1‑76 (MQ=255) aagaagGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCaa > 2:609809/1‑76 (MQ=255) aagaagGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCaa < 2:402884/76‑1 (MQ=255) agaagGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAAc < 1:39784/76‑1 (MQ=255) agGGGATGATGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCg < 2:1331825/76‑1 (MQ=255) gatgatGTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGa > 1:345962/1‑76 (MQ=255) atgatgTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGAc < 2:1178671/76‑1 (MQ=255) gatgTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCg < 2:890112/76‑1 (MQ=255) atgTAGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGCCCgg > 2:1233261/1‑76 (MQ=255) aGCACTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTaaa > 2:585594/1‑76 (MQ=255) cTGGTAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAgaga < 2:250583/76‑1 (MQ=255) tAGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCAc > 1:44434/1‑76 (MQ=255) aGGTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCAcc < 1:678071/76‑1 (MQ=255) ggTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCa > 1:264838/1‑76 (MQ=255) ggTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCa < 1:960481/76‑1 (MQ=255) gTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCAt > 1:800624/1‑76 (MQ=255) gTTTTGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCAt < 1:85653/76‑1 (MQ=255) tttGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCg > 2:1361870/1‑76 (MQ=255) tGGTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCgct > 2:891419/1‑76 (MQ=255) ggTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCgctg > 2:83818/1‑76 (MQ=255) gTACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCgctgc > 1:904872/1‑76 (MQ=255) tACTGTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCgctgct > 1:1331575/1‑76 (MQ=255) | ATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGCCGTTAAAGAGCGTGCTGCCCGTACTGGCCGCAACCCGCAGACCGGTAAAGAGATCACCATCGCTGCT > REL606/433582‑433724 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |