| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,162,470 | A→C | T539P (ACC→CCC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,162,470 | 0 | A | C | 97.6% | 165.0 / ‑4.0 | 41 | T539P (ACC→CCC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (20/20); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01 | |||||||||||
ATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCG > REL606/4162398‑4162539 | aTGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGcccc > 1:1462001/1‑76 (MQ=255) tGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCg > 2:1390191/1‑76 (MQ=255) ccAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGaa < 1:46967/76‑1 (MQ=255) cAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAAc > 1:897898/1‑76 (MQ=255) aacaacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCTGCCCAGGCGGTCTGCCCCGTGAACGTg < 2:887288/76‑1 (MQ=255) aacaacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTg < 1:1118017/76‑1 (MQ=255) accccGCGGTGTGAGAGTACGCACAAGCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAg < 2:150282/74‑1 (MQ=255) aacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAg > 2:264725/1‑76 (MQ=255) aacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAg > 1:1059559/1‑76 (MQ=255) acCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAgg < 2:374901/76‑1 (MQ=255) acCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAgg < 1:400746/76‑1 (MQ=255) acCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAgg < 1:526155/76‑1 (MQ=255) cccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGc > 2:90169/1‑76 (MQ=255) gCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTc < 1:721815/76‑1 (MQ=255) tGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGa < 1:109740/76‑1 (MQ=255) tGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGa > 1:729367/1‑76 (MQ=255) gTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGaa > 2:1487328/1‑76 (MQ=255) tCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAg < 1:1301011/76‑1 (MQ=255) cTGAGATTACGCACAAACGTCGTATCTCCGCAGTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGt < 1:581737/76‑1 (MQ=255) aTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCgag > 2:1119491/1‑76 (MQ=255) aTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCgag < 1:1326337/76‑1 (MQ=255) tACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGAc < 2:842415/76‑1 (MQ=255) aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACg < 1:33469/76‑1 (MQ=255) aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACg > 1:235125/1‑76 (MQ=255) gCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTa < 1:428811/76‑1 (MQ=255) gCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTa < 1:208919/76‑1 (MQ=255) cacaAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTac > 1:517055/1‑76 (MQ=255) caAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTacac > 2:1401496/1‑76 (MQ=255) aaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACAccc < 2:480584/76‑1 (MQ=255) cgtATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTc < 1:450709/76‑1 (MQ=255) cgtATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTc > 1:1308098/1‑76 (MQ=255) cgtATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTc > 2:62534/1‑76 (MQ=255) gtATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCa > 2:978925/1‑76 (MQ=255) tATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCAc > 2:500361/1‑76 (MQ=255) tATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCAc > 2:902865/1‑76 (MQ=255) ctcCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACAACCGACTCACTAc > 1:516502/1‑76 (MQ=255) cccGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACg < 1:1429339/75‑1 (MQ=255) cACTCGGCCCAGTCGGGCTGCCCCCCAAACGAGCAGGCTTCGAAGCTCGAGACGCCCACCCCACTCACTACGGTcg > 2:615970/1‑76 (MQ=255) gCCCGGTCGGTCTGCCCGGTTGGCGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATg < 2:465039/76‑1 (MQ=255) cAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTcc < 1:1013417/76‑1 (MQ=255) ggCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCaa > 2:642550/1‑76 (MQ=255) ggCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCaa > 1:343843/1‑76 (MQ=255) gCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAAt < 1:1271051/76‑1 (MQ=255) cGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACTCCCGACTCACTACGGTCGCGTATGTCCAATc < 1:214276/76‑1 (MQ=255) ggTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCg > 2:148567/1‑76 (MQ=255) | ATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCG > REL606/4162398‑4162539 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |