| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,237,516 | (T)7→6 | intergenic (+41/+62) | proL → / ← yejO | tRNA‑Pro/predicted autotransporter outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,237,510 | 0 | T | . | 96.0% | 106.3 / ‑2.4 | 25 | intergenic (+35/+68) | proL/yejO | tRNA‑Pro/predicted autotransporter outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (8/16); total (8/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAG > REL606/2237441‑2237581 | gggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGtttttta > 1:599191/1‑75 (MQ=255) gTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATc > 2:276035/1‑76 (MQ=255) cGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTg < 2:32102/76‑1 (MQ=255) ggAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGc < 1:291224/76‑1 (MQ=255) ggAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGc > 2:167094/1‑76 (MQ=255) aGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCaa < 2:260091/76‑1 (MQ=255) gTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCaatt < 2:47304/76‑1 (MQ=255) aaaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCaattaatt < 2:462062/76‑1 (MQ=255) aaaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCaattaatt < 2:140633/76‑1 (MQ=255) tctcGTGCCTACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATa < 1:43978/76‑1 (MQ=255) tctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATTTGCAATTAATTCGATaa > 2:272749/1‑76 (MQ=255) ctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACTGTTGG‑TTTTTTATATCTGCAATTAATTCGATaaa < 2:157299/76‑1 (MQ=255) tcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAAc < 1:111958/76‑1 (MQ=255) cGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACa < 1:497103/76‑1 (MQ=255) tGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGa > 2:631810/1‑76 (MQ=255) aCCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTg < 2:119675/76‑1 (MQ=255) aCCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTg > 2:275589/1‑76 (MQ=255) aaaaTCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGacac > 2:467808/1‑76 (MQ=255) aaaTCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGAcaca < 2:413134/76‑1 (MQ=255) ccAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATcaca < 1:343166/76‑1 (MQ=255) aaGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGc > 1:481283/1‑76 (MQ=255) aaaaaaCCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTg < 1:375784/76‑1 (MQ=255) aaaaaCCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGt < 2:502585/76‑1 (MQ=255) ttACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGtt > 1:52597/1‑76 (MQ=255) ttACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGtt < 1:397123/76‑1 (MQ=255) ttACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGtt < 1:397113/76‑1 (MQ=255) ttACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGtt < 2:432959/76‑1 (MQ=255) ggttgg‑ttTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCa < 1:189430/76‑1 (MQ=255) gttgg‑ttTTTTATATCTGCAATTAATTCGATAAACAGACCGTTACACATCACAGCCTGTTTATTTTCTGTTATCAg < 1:87836/76‑1 (MQ=255) gttgg‑ttTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAg < 1:202747/76‑1 (MQ=255) | GGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAG > REL606/2237441‑2237581 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |