Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	3,894,997	Δ6,934 bp	IS150‑mediated	rbsD–[yieO]	rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	3893623–3894994	3901930	6937–8308	6 [4]	[0] 14	[insJ‑5]–[yieO]	[insJ‑5],insK‑5,rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 3652533	NA (NA)	14 (0.670)	11/146	0.9	100%	noncoding (1443/1443 nt)	IS150	repeat region
	?	REL606	3901931 =	0 (0.000)					coding (489/1428 nt)	yieO	predicted multidrug or homocysteine efflux system

GCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGA  >  REL606/3893547‑3893626                                                                            |     gCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtt      <  1:270720/76‑1 (MQ=39) gCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtt      <  1:465733/76‑1 (MQ=39)   cAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtttt    >  2:412136/1‑76 (MQ=35)    aGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTg   <  1:186626/76‑1 (MQ=34)    aGACTGTACTGCACCCATTTTGTTGGACGATCAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTg   <  1:186622/76‑1 (MQ=21)     gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa  <  1:551206/76‑1 (MQ=32)                                                                            |     GCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGA  >  REL606/3893547‑3893626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑