| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,499,896 | T→C | E303G (GAG→GGG) | glgC ← | glucose‑1‑phosphate adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,499,896 | 0 | T | C | 90.9% | 35.7 / ‑2.9 | 11 | coding (908/1317 nt) | glgC | glucose‑1‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (4/6); minor base . (1/0); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
CTGCACGAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTA > REL606/3499823‑3499966 | cTGCACGAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGcccc > 2:524851/1‑76 (MQ=255) tttCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCTATTGCGATCGTACATATCCAGCCCCGGCACcaca < 2:177348/76‑1 (MQ=255) ttCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCCCCGGCACCACag < 1:318691/76‑1 (MQ=255) aTTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCCCCGGCACCACAGAGGCCAGATCGAGGtt < 2:286936/76‑1 (MQ=255) tGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGC‑CCCGCCCCACAGAGGCCAGAATGAGGGTCCCTTTc > 2:495463/1‑76 (MQ=255) gTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCCCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTc > 2:515443/1‑76 (MQ=255) aGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCCCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCa < 2:501055/76‑1 (MQ=255) gCGAATTGGCCAATTGCGATCGTACATATCCAGACCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTaa < 1:18129/76‑1 (MQ=255) aaTTGCGATCGTACATATCCAGCCCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGt > 1:487966/1‑76 (MQ=255) tACATATCCAGCCCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATcgc < 1:429173/76‑1 (MQ=255) atatCCAGCCCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGcc > 1:645317/1‑76 (MQ=255) ccAGCCCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTa > 1:596153/1‑76 (MQ=255) | CTGCACGAATTTCGCTGGCGGTAATGATTCATTGTAGGTGCGAATTGGCCAATTGCGATCGTACATATCCAGCTCCGGCACCACAGAGGCCAGATCGAGGTTCGCTTTCCAGTAAGCTTCCAGCGTACCCACATCGCGCCAGTA > REL606/3499823‑3499966 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |