Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,305,498	A→C	F248V (TTC→GTC)	cls ←	cardiolipin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,305,498	0	A	C	100.0%	58.5 / NA	15	F248V (TTC→GTC)	cls	cardiolipin synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (7/8);  total (7/8)

ATGGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCG  >  REL606/1305437‑1305567                                                              |                                                                      gggggcCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGACGTAGCGAGGGTCGa                                                         <  2:328191/74‑1 (MQ=255)    gggCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGACGTAGCGAGGGTCGACCa                                                      >  1:169013/1‑76 (MQ=255)         ttCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGACGTAGCGAGGGTCGACCATATTc                                                 <  1:95898/76‑1 (MQ=255)          tCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGACGTAGCGAGGGTCGACCATATTCa                                                >  1:236813/1‑76 (MQ=255)                  cgcCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGACGTAGCGAGGGTCGACCATATTCATGCTGCCg                                        <  1:607602/76‑1 (MQ=255)                     cATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGACGTAGCGAGGGTCGACCATATTCATGCTGCCGgtg                                     >  1:404419/1‑76 (MQ=255)                      aTCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGACGTAGCGAGGGTCGACCATATTCATGCTGCCGgtgt                                    <  2:164597/76‑1 (MQ=255)                           aTCAATCCATTGCCCTACGCCCGCATCTTGTTTGACGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTAcgcg                               <  1:175379/76‑1 (MQ=255)                                tCCATTGCCCTACGCCCGCATCTTGTTTGACGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTa                          >  1:373859/1‑76 (MQ=255)                                       cccTACGCCCGCATCTTGTTTGACGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCg                   >  1:150126/1‑76 (MQ=255)                                          tACGCCCGCATCTTGTTTGACGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatc                <  1:560895/76‑1 (MQ=255)                                          tACGCCCGCATCTTGTTTGACGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatc                >  2:174485/1‑76 (MQ=255)                                             gCCCGCATCTTGTTTGACGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatcatg             <  1:244402/76‑1 (MQ=255)                                                  cATCTTGTTTGACGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatcatgatcat        <  2:140074/76‑1 (MQ=255)                                                        gTTTGACGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCg  >  1:203428/1‑76 (MQ=255)                                                              |                                                                      ATGGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCG  >  REL606/1305437‑1305567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑