Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,894,119	C→A	intergenic (‑8/+89)	proQ ← / ← yebR	putative solute/DNA competence effector/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,894,119	0	C	A	100.0%	76.5 / NA	22	intergenic (‑8/+89)	proQ/yebR	putative solute/DNA competence effector/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/8);  total (14/8)

AAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTACC  >  REL606/1894049‑1894183                                                                       |                                                                 aaaaCGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGAt                                                             >  2:248925/1‑76 (MQ=255)   aaCGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTa                                                           >  1:359229/1‑76 (MQ=255)          ggCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGa                                                    >  2:25131/1‑76 (MQ=255)              aGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTa                                                >  1:670839/1‑76 (MQ=255)               gAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAg                                               >  1:168384/1‑76 (MQ=255)                  aCGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCca                                            <  2:133678/76‑1 (MQ=255)                  aCGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCca                                            <  1:567841/76‑1 (MQ=255)                      gATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCcaacaa                                        >  1:339757/1‑76 (MQ=255)                        ttACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAgc                                      >  1:206344/1‑76 (MQ=255)                        ttACTTCTTTACTGCTATTCAAATTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAgc                                      >  2:546375/1‑76 (MQ=255)                          aCTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAgcgc                                    <  1:550186/76‑1 (MQ=255)                                 tACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATg                             >  2:329689/1‑76 (MQ=255)                                  aCTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGa                            <  1:631332/76‑1 (MQ=255)                                  aCTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGa                            <  2:523228/76‑1 (MQ=255)                                    tGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAAc                          <  1:264426/76‑1 (MQ=255)                                     gCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACa                         >  2:630995/1‑76 (MQ=255)                                                tAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATtat              >  2:223287/1‑76 (MQ=255)                                                tAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATtat              >  2:341397/1‑76 (MQ=255)                                                 aGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATtata             <  1:373870/76‑1 (MQ=255)                                                   gTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAAt           <  1:31319/76‑1 (MQ=255)                                                         tttCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCt     >  1:557048/1‑76 (MQ=255)                                                            ccATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTAcc  >  2:484697/1‑76 (MQ=255)                                                                       |                                                                 AAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTACC  >  REL606/1894049‑1894183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑