Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	474,520	Δ1 bp	coding (786/1677 nt)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	474,520	0	G	.	100.0%	203.8 / NA	41	coding (786/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (23/18);  total (23/18)

AAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCAAACAGCTCTACCGCACCAAAGG  >  REL606/474451‑474590                                                                      |                                                                       aaaCAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTa                                                                 >  2:860479/1‑76 (MQ=255) aaaCAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTa                                                                 >  1:528104/1‑76 (MQ=255)  aaCAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTAc                                                                <  1:974555/76‑1 (MQ=255)    cAGCGCCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCa                                                              <  2:541597/76‑1 (MQ=255)     aGCACCGCAAACGCGTCGCGCAATGGCAGCGTGTCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCAt                                                             <  1:269394/76‑1 (MQ=255)       cACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATcc                                                           <  2:290887/76‑1 (MQ=255)          cGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCgg                                                        >  2:1143033/1‑76 (MQ=255)             aaaCGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCAGCaa                                                     >  2:1016776/1‑76 (MQ=255)                  cgTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAAc                                                >  2:1043102/1‑76 (MQ=255)                   gTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACg                                               <  1:464684/76‑1 (MQ=255)                   gTCGCGCAATGACAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACg                                               >  1:520899/1‑76 (MQ=255)                    tCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGc                                              >  2:235927/1‑76 (MQ=255)                        gcAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCg                                          >  2:172898/1‑76 (MQ=255)                          aaTGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAg                                        >  2:635343/1‑76 (MQ=255)                             ggCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGc                                     >  1:427990/1‑76 (MQ=255)                              gCAGCGTCTCGTGGGCGGCACGGTGACTCACTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCa                                    <  1:21480/76‑1 (MQ=255)                              gCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCa                                    <  1:1047341/76‑1 (MQ=255)                               cAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCaa                                   >  1:294820/1‑76 (MQ=255)                               cAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCaa                                   >  1:269613/1‑76 (MQ=255)                                aGCGTATCGTGGGCGGCGCGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCaaa                                  >  1:1332042/1‑76 (MQ=255)                                aGCGTATCGTGGGCGGCACGATGACTCAGTTCAGACTC‑TTCAGTACCATCCCGACAAAGAACGCACCGAGTGCaaa                                  >  2:108790/1‑76 (MQ=255)                                   gTATCGTGGGCNGCNCGGTGACTCAGCTCAGACTC‑TTCAGTACCATCCCGGCAAAGAGAGCACCGAATGCAAAGga                               <  2:205387/76‑1 (MQ=255)                                     aTCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGgaga                             >  1:129684/1‑76 (MQ=255)                                      tCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGAc                            <  1:480140/76‑1 (MQ=255)                                      tCGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGAc                            <  2:306920/76‑1 (MQ=255)                                       cGTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACa                           >  1:472250/1‑76 (MQ=255)                                        gTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAANAACGCACCGAGTGCAAAGGAGACAt                          >  2:91767/1‑76 (MQ=255)                                        gTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACAt                          <  1:960723/76‑1 (MQ=255)                                        gTGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACAt                          <  1:233651/76‑1 (MQ=255)                                        gTGGGCGGCACGGTGACTCAGTTAAGCNTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACAt                          >  2:3476/1‑76 (MQ=255)                                         tGGGCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATc                         >  2:762100/1‑76 (MQ=255)                                          gggCGGCACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCa                        >  2:304568/1‑76 (MQ=255)                                           ggcggcACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCaa                       <  1:1016704/76‑1 (MQ=255)                                                cACGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCAAACAGc                  >  2:474250/1‑76 (MQ=255)                                                  cGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCAAACAGctc                >  2:373808/1‑76 (MQ=255)                                                  cGGTGACTCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCAAACAGctc                <  1:914026/76‑1 (MQ=255)                                                         tCAGTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCAAACAGCTCTACCGCa         <  2:816288/76‑1 (MQ=255)                                                            gTTCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCAAACAGCTCTACCGCACCa      <  1:1024955/76‑1 (MQ=255)                                                              tCAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCAAACAGCTCTACCGCACCaaa    <  2:717441/76‑1 (MQ=255)                                                               cAGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCAAACAGCTCTACCGCACCAAAg   >  2:1224011/1‑76 (MQ=255)                                                                aGACTC‑TTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCAAACAGCTCTACCGCACCAAAgg  <  2:943685/76‑1 (MQ=255)                                                                      |                                                                       AAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACGCACCGAGTGCAAAGGAGACATCAAACAGCTCTACCGCACCAAAGG  >  REL606/474451‑474590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑