| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,893,551 | +G | intergenic (+6/‑50) | kup → / → insJ‑5 | potassium transporter/IS150 hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,893,550 | 1 | . | G | 95.3% | 155.9 / 0.4 | 43 | intergenic (+5/‑51) | kup/insJ‑5 | potassium transporter/IS150 hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (24/17); total (26/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
TGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCA‑GACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACC > REL606/3893475‑3893619 | tGCGTGCACCAGATCGATTTGAAATCCCGCCAATCAGGGTTATCGAACTGGGTACTCAGGTNGAAATCTAACG‑CCa > 1:1153291/1‑76 (MQ=255)tGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCa > 1:863095/1‑76 (MQ=255) gCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCA‑g < 1:857365/76‑1 (MQ=255) gTGCACCAGATCAATTTGACATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCA‑gga > 2:749953/1‑74 (MQ=255) tGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAATN‑CCA‑ggac < 2:1084454/76‑4 (MQ=255) aaTTTGAAATCCCGCCACACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAGCG‑CCAGGACTGTACTGCAcc > 1:1325205/1‑76 (MQ=255) aaaTCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCAtttt > 2:93334/1‑76 (MQ=255) cccGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTttgttg < 1:150977/76‑1 (MQ=255) cGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGa < 1:882792/76‑1 (MQ=255) gCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGAc > 1:337498/1‑76 (MQ=255) gCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGCACTGCACCCATTTTGTTGGAc > 1:421594/1‑76 (MQ=39) ccAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACg < 1:693866/76‑1 (MQ=255) cAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGa > 1:671915/1‑76 (MQ=255) aaaCAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGAt > 2:631543/1‑76 (MQ=255) aGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAAGGCCCG‑GACTGTACTGCACTCATTTTGTTGGACGATGaaa > 2:1059051/1‑76 (MQ=25) aGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGaaa > 1:740134/1‑76 (MQ=255) ggTTATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATg < 2:244661/76‑1 (MQ=255) ttATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGa < 2:301496/76‑1 (MQ=255) ttATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGa > 1:824522/1‑76 (MQ=255) ttATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCA‑GACTGTACTGCACCCATTTTGTTGGACGATGAAATGGaa > 2:135209/1‑76 (MQ=255) tATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGaa > 2:502182/1‑76 (MQ=255) aTCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAAt < 1:1222774/76‑1 (MQ=255) tCGAATGGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGCTGAAATGGAATa < 2:56562/76‑1 (MQ=21) tCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATa < 1:1109948/76‑1 (MQ=255) tCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATa < 2:1220117/76‑1 (MQ=255) tCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATa < 2:1220123/76‑1 (MQ=255) tCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATa < 2:384021/76‑1 (MQ=255) cGAACTGGGTACTCATGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAg > 2:719792/1‑76 (MQ=21) cGAACTGGGTACTCACGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAg > 2:719776/1‑76 (MQ=21) gAACTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGc > 2:1044491/1‑76 (MQ=255) aCTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGccc > 2:749273/1‑76 (MQ=255) cTGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGcccc > 2:777881/1‑76 (MQ=255) tGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCt > 1:140537/1‑76 (MQ=255) tGGGTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCt < 2:1174168/76‑1 (MQ=255) ggTACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTaa > 1:864774/1‑76 (MQ=255) tACTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAata < 2:741055/76‑1 (MQ=255) aCTCAGGTCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAatat > 2:245831/1‑76 (MQ=255) tCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg < 2:159366/76‑1 (MQ=255) tCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg > 2:841337/1‑76 (MQ=255) tCGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg > 1:944495/1‑76 (MQ=255) cGAAATCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGc > 2:32832/1‑76 (MQ=255) naaTCTAACG‑CCAGGACTGTACTGCACCCATTTTCTTNGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa > 1:1308885/2‑76 (MQ=14) aaaTCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa < 2:415223/76‑1 (MQ=255) aaaTCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa < 1:380245/76‑1 (MQ=255) aaaTCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa > 2:1007810/1‑76 (MQ=255) aaaTCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa > 2:781267/1‑76 (MQ=255) tCTAACG‑CCAGGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaaaa > 1:439395/1‑76 (MQ=255) aCG‑CCAGGACTGTACTGTAGCCTTTTTGTTGGACGATGAAATGGAATAGCCGCTAGTATGTCAAAGCCAAAATAcc < 1:64603/76‑1 (MQ=11) | TGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAACG‑CCA‑GACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACC > REL606/3893475‑3893619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |