| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,747,903 | 0 | G | T | 34.2% | 127.8 / 73.6 | 76 | A8S (GCA→TCA) | ydiL | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (38/12); new base T (26/0); total (64/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.11e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAA > REL606/1747829‑1747974 | agGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGc > 2:896619/1‑76 (MQ=255) ggNTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAATTCCATNCa < 2:1092123/76‑1 (MQ=255) ggCTGATAATGTTATTTACATNTTNTTGTTTATTTCATGCAGCCATNACACGATNANCGCTTATGAACTCCAGGCa > 2:193210/1‑76 (MQ=255) ggCTGATAAGGATATTTATATTTTATTGTTTATATCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCa > 1:78820/1‑76 (MQ=255) gCTGATAATGTTATTTATCTTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:1265318/1‑76 (MQ=255) gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:370245/1‑76 (MQ=255) gCTGATAATGATATTTACATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAc > 1:109094/1‑76 (MQ=255) cTGATAATNTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACTCTTATGAACTCCAGTCACt > 1:1292402/1‑76 (MQ=255) cTGATAATGTTATTTATNTTTTATTGTTTATGTCATGCAGCCATTACACGATGAACGCTTATGCACACCAGTCACt > 1:170359/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcg > 1:373596/1‑75 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACt > 1:573539/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACt > 1:1073649/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACt > 1:679858/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACt > 1:693768/1‑76 (MQ=255) cTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACt > 1:723522/1‑76 (MQ=255) cTGATAATGCTCTTTATATCTTATTGTTTATNTGATTCCCCCATTACACGATGAACGCTTATGCNCTCCAGTCACt > 1:163720/1‑76 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCNTTACACGATGAACGCTTANGANCTCCAGTCAcca > 1:1086810/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCCTTACACGATGAACGCTTATGAACTCCAGTCACtt > 1:1107777/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcct > 1:384901/1‑74 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtt > 1:678011/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtt > 1:617756/1‑75 (MQ=255) tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtt > 1:499056/1‑75 (MQ=255) tGATAATGTTAGTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACta > 1:39955/1‑75 (MQ=255) gatgatATTATGTATATTTTATTGTTTATTTCACGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:1224214/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACGCGATGAACGCTTATGAACTCcactcactta > 1:27454/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcatc > 1:771687/1‑73 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCAcatc > 1:149945/1‑73 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:945876/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:393529/1‑74 (MQ=255) gATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACtta > 1:696734/1‑74 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACttag > 1:1054650/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGTCACttag > 1:221728/1‑73 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCg > 2:671798/1‑76 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCg > 1:145982/1‑76 (MQ=255) aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCg > 2:1273507/1‑76 (MQ=255) aTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCa > 1:966479/1‑76 (MQ=255) aTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCa > 1:966480/1‑76 (MQ=255) tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCat > 1:958463/1‑76 (MQ=255) tGTTATTTATATGCTGTGACTGATTCCATGCAGCCATTACGCGCTGAACGCTTATGAACTCCAGACACTTAGCCat > 1:15213/1‑76 (MQ=255) gTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCcta > 2:586608/1‑76 (MQ=255) gTTATTTATATTTTATTGTTTATTTCATCCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCata > 2:1204149/1‑76 (MQ=255) ttatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCatat > 2:1001992/1‑76 (MQ=255) tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtt > 2:344479/1‑76 (MQ=255) tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAtt < 2:454703/76‑1 (MQ=255) atttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATAttt > 2:184457/1‑76 (MQ=255) tataTTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTg > 2:183258/1‑76 (MQ=255) tatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGcc > 2:1114500/1‑76 (MQ=255) ttttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCAt < 1:312404/76‑1 (MQ=255) ttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGa < 1:347246/76‑1 (MQ=255) ttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGa > 2:1148503/1‑76 (MQ=255) ttATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGa > 1:390932/1‑76 (MQ=255) ttATTGTTCATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGa > 1:1246156/1‑76 (MQ=255) tttGTTTTTTTCAGGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACt < 1:782061/75‑1 (MQ=255) aTTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACt > 2:622771/1‑76 (MQ=255) ttttttATTCCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTa < 1:1078277/73‑1 (MQ=255) ttGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTa < 2:1228564/76‑1 (MQ=255) tGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAt > 2:451743/1‑76 (MQ=255) gTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAtt > 2:1198969/1‑76 (MQ=255) tttATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtg > 2:351239/1‑76 (MQ=255) tttATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtg > 1:952222/1‑76 (MQ=255) ttATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga > 1:764711/1‑76 (MQ=255) tATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgat > 1:850462/1‑76 (MQ=255) tATNTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgat > 1:1205188/1‑76 (MQ=255) aTTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtgatg > 1:795228/1‑76 (MQ=255) tttCATGCNGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATagatga > 1:1282151/1‑76 (MQ=255) cATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGACTg > 2:1251298/1‑76 (MQ=255) atccAGCCATTACACGATGAACGCTTATGAACCCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgc < 1:697942/73‑1 (MQ=255) tGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcg > 2:75833/1‑76 (MQ=255) tGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcg > 2:750546/1‑76 (MQ=255) gCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcgc > 2:1275995/1‑76 (MQ=255) gCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcgc > 2:895947/1‑76 (MQ=255) ccATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAcc < 2:424699/76‑1 (MQ=255) cATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCt > 2:78621/1‑76 (MQ=255) cacGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAtt < 2:807063/76‑1 (MQ=255) acGATGAACGCTTATGAACTCCAGGCACTCCGCCATATNTTGGCCATGACTATTGATGAATGCGCGACCTGGATTg < 1:1309190/76‑1 (MQ=255) aTGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGccc < 2:488808/76‑1 (MQ=255) agAACGCTTAAGAACCCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCa < 2:566443/75‑1 (MQ=255) aaCGCTTATGAACTCCAGGCACTCCGCCATATTTTTTCCATGACTATTNATGAATGCGCGACCTGGATTGCCCAGa < 1:1176649/76‑1 (MQ=255) cGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGCCCTGGATTGCCCAGAcc > 1:431382/1‑76 (MQ=255) cGCTTATGAACNCCNAGCACTCCGCCTTATTTTTGCCATGACTATTGATGAATGCGCTACCTGGATGGCCCAGAcc < 2:203884/76‑1 (MQ=255) cTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCgg < 1:517798/76‑1 (MQ=255) aaCTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAg < 1:1029361/76‑1 (MQ=255) aCTCCAGGCACTCCGCCCTATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGt > 2:471250/1‑76 (MQ=255) aCTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGt > 2:471260/1‑76 (MQ=255) ccAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGaa > 2:497215/1‑76 (MQ=255) | AGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAA > REL606/1747829‑1747974 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |