| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,027,586 | C→T | A29T (GCC→ACC) | ECB_02827 ← | Polysialic acid transport ATP‑binding KpsT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,027,586 | 0 | C | T | 97.8% | 169.8 / ‑3.5 | 46 | A29T (GCC→ACC) | ECB_02827 | Polysialic acid transport ATP‑binding KpsT |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (20/25); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01 | |||||||||||
CTGTCGGGGCGGTCAATACCACCAATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGCAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTAAACACATAGTGTCGCCCCGTTGGGGTACGATATGA > REL606/3027513‑3027661 | cTGTCGGGGCGGTCAATACCACCAATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTaa < 2:476237/76‑1 (MQ=255) gTCGGGGCGGTCAATACCACCAATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACg < 2:683415/76‑1 (MQ=255) tCGGGGCGGTCAATACCACCAATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGc > 2:1052527/1‑76 (MQ=255) gggCGGTCAATACCACCAATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCttt > 1:675017/1‑76 (MQ=255) cGGTCAATACCACCAATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCCTTTTc > 2:1001107/1‑76 (MQ=255) ggTCAATACCACCAATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGCAACGCTTTTTcc > 1:566623/1‑76 (MQ=255) gTCAATACCACCAATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCt > 1:343822/1‑76 (MQ=255) gTCAATACCACCAATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCt < 2:378931/76‑1 (MQ=255) tCAATACCACCAATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTg > 2:804080/1‑76 (MQ=255) aaTACCACCAATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAATGGTAACGCTTTTTCCTGaa < 1:1139721/76‑1 (MQ=255) aaTACCACCAATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGaa < 1:266511/76‑1 (MQ=255) tACCACCGATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAgg < 2:143285/76‑1 (MQ=255) tACCACCAATCATTCTTAGCAACGGTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAgg < 2:143308/76‑1 (MQ=255) ccaccaATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGa < 2:576325/76‑1 (MQ=255) caATCATTCTTAGCAACGTTGATTTACNCGCACCGTCGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTc > 1:1178434/1‑76 (MQ=255) caATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTc > 1:605655/1‑76 (MQ=255) caATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTc > 1:709035/1‑76 (MQ=255) aaTCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCg < 2:1222457/76‑1 (MQ=255) ttCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGtt < 2:100639/76‑1 (MQ=255) ttCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGtt > 2:1282870/1‑76 (MQ=255) ttAGCTACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTaa < 2:50402/76‑1 (MQ=255) ttAGCAACGTTGATGTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAGGGGATTGCGAGGTTTaa < 1:106612/76‑1 (MQ=255) tAGCAATGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTNaaa < 1:1198312/76‑1 (MQ=255) aGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTTAAt < 1:831084/76‑1 (MQ=255) aGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAAt < 1:831097/76‑1 (MQ=255) cGTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTa > 1:768485/1‑76 (MQ=255) gTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTaa > 1:723881/1‑76 (MQ=255) gTTGATTTACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTaa > 1:723872/1‑76 (MQ=255) tttACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTAAAcaca < 1:822300/76‑1 (MQ=255) tttACCCGCACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTAAAcaca < 2:181027/76‑1 (MQ=255) tACCCGCACCGTTGAGACCGATGAATGTAACGCTTTTTCCTGAAGGGATTTAGAGGTTTAAATCCTTAAACACATa < 1:64509/76‑1 (MQ=255) gCACCGTTGCGACCNATAAAGGTAACGCTTTTTCCTGAAGGGCTTTCGAGGTTTAAATCCATAAACACATAGTGTc < 1:1142621/76‑1 (MQ=255) cACCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTAAACACATAGTGTCg < 2:891545/76‑1 (MQ=255) cACCGTTGCGACCAATAAAGGTAACGCTTTTTCATGAAGGGATTTCGAGGTTTAAATCCTTAAACACATAGTGTCg > 2:719816/1‑76 (MQ=255) cACCGTTGCGACCAATAAAGATAACGCTTTTTCCTTAAGGGATTTCGAGGTTTAAATCCTTAAACACATAGTGTCg < 2:1058864/76‑1 (MQ=255) aCCGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTAAACACATAGTGTCGc > 2:1293063/1‑76 (MQ=255) ccGTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTAAACACATAGTGTCGcc > 2:69375/1‑76 (MQ=255) gTTGCGACCAATAAAGGTAACGCTTTTTCCTGAAGAGATTTCGAGGTTTAAATCCTTAAACACATAGTGTCGcccc > 1:363074/1‑76 (MQ=255) ttGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTAAACACATAGTGTCGCCCCg < 2:877532/76‑1 (MQ=255) ttGCGACCAATAAAGGTAACGCTTTTTACTGAAGNGATTTCGNTGNTTNAATCATTATACACATAGTGTCGCCCCg > 1:1160624/1‑76 (MQ=255) tGCGACCAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTAAACACATAGTGTCGCCCCGt > 2:958249/1‑76 (MQ=255) cAATAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTAAACACATAGTGTCGCCCCGTTGGGGt > 1:99712/1‑76 (MQ=255) aTAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTAAACACATAGTGTCGCCCCGTTGGGGTAc < 1:151421/76‑1 (MQ=255) aTAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTAAACACATAGTGTCGCCCCGTTGGGGTAc < 2:341481/76‑1 (MQ=255) aTAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTAAAACACATAGTGTCGCCCCGTTGGGGTAc > 2:24615/1‑76 (MQ=255) tAAAGGTAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTAAACACATAGTGTCGCCCCGTTGGGGTACg < 2:856110/76‑1 (MQ=255) cAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTAAACACATAGTGTCGCCCCGTTGGGGTACGATATGa < 2:1156291/76‑1 (MQ=255) | CTGTCGGGGCGGTCAATACCACCAATCATTCTTAGCAACGTTGATTTACCCGCACCGTTGCGACCAATAAAGGCAACGCTTTTTCCTGAAGGGATTTCGAGGTTTAAATCCTTAAACACATAGTGTCGCCCCGTTGGGGTACGATATGA > REL606/3027513‑3027661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |