Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,096,436	C→T	A136T (GCA→ACA)	sufI ←	repressor protein for FtsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,096,436	0	C	T	100.0%	108.5 / NA	31	A136T (GCA→ACA)	sufI	repressor protein for FtsI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/12);  total (19/12)

ATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCA  >  REL606/3096363‑3096507                                                                          |                                                                        aTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTgtgt                                                                       <  1:350723/76‑1 (MQ=255) aTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTgtgt                                                                       <  1:350737/76‑1 (MQ=255)   tCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTc                                                                     >  1:1024861/1‑76 (MQ=255)          aGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATTCCACAGTGTAGCTGTGTTCTGGCGac                                                              >  2:666196/1‑75 (MQ=255)            gCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATg                                                            <  2:1163835/76‑1 (MQ=255)             ccGTTATAGACCTGCTGAGCCGTGAGGTNCGGAGTATTGGCGTGATACTANAGAGTAGCTGTGTTCTGGCGAATgg                                                           >  1:193161/1‑76 (MQ=255)              cGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATggg                                                          >  2:1219697/1‑76 (MQ=255)               gTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTATTCTGGCGAATGGGc                                                         >  1:1101411/1‑76 (MQ=255)                ttATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCa                                                        >  1:689242/1‑76 (MQ=255)                ttATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCa                                                        >  2:749297/1‑76 (MQ=255)                  ataGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGt                                                      <  2:1194665/76‑1 (MQ=255)                  ataGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGt                                                      >  1:365611/1‑76 (MQ=255)                   taGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTa                                                     <  2:1281802/76‑1 (MQ=255)                        ctgctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCCAATGGGCAGTACGGGt                                                >  1:476953/1‑76 (MQ=255)                         tgctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTg                                               <  2:279814/76‑1 (MQ=255)                          gctgAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGc                                              >  1:663417/1‑76 (MQ=255)                             gAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCa                                           >  1:1043278/1‑76 (MQ=255)                              aGCTGTGCAGTTGGGAGTATTGGCGTGATACCACAGAGCAGCAGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAg                                          <  2:871562/76‑1 (MQ=255)                              aGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTTGCGAATGGGCAGTACGGGTGCCCAg                                          <  2:718442/76‑1 (MQ=255)                                ccGTGCGGTTGGNAGNATTGGCGTGATACCACAGNGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTc                                        <  1:1093852/76‑1 (MQ=255)                                 cGTGCGGTTGGGAGTGTTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCa                                       >  1:126084/1‑76 (MQ=255)                                   tGCGGGTGGGAGTATTGGCGTGATACCACAGAGTAGCGATGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGc                                     <  1:1114183/76‑1 (MQ=255)                                           ggANTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCg                             >  1:1199889/1‑76 (MQ=255)                                             aGTATTGGCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAc                           >  2:631747/1‑76 (MQ=255)                                                   ggCGTGATGCCACAGAGTAGCTGTGTTCTGGCGGATGGTCAGTACGGGTGCCCAGTCAGCGTTTGGCGACatcatc                     <  1:381292/76‑1 (MQ=255)                                                   ggCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACatcatc                     <  2:582228/76‑1 (MQ=255)                                                   ggCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACatcatc                     >  2:911887/1‑76 (MQ=255)                                                   ggCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACatcatc                     <  1:352274/76‑1 (MQ=255)                                                    gCGTGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGAAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATcc                    <  1:33988/76‑1 (MQ=255)                                                       tGATACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTg                 <  1:992835/76‑1 (MQ=255)                                                         aTACCACTGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGcc               <  2:1128608/76‑1 (MQ=255)                                                         aTACCACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGcc               >  1:745832/1‑76 (MQ=255)                                                            ccACAGAGTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGa            >  2:336258/1‑76 (MQ=255)                                                                  agTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGccc      >  2:317657/1‑76 (MQ=255)                                                                   gTAGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCa     >  1:403594/1‑76 (MQ=255)                                                                     aGCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATc   >  1:931845/1‑76 (MQ=255)                                                                      gCTGTGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCa  <  2:61420/76‑1 (MQ=255)                                                                          |                                                                        ATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCGCCCATCA  >  REL606/3096363‑3096507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑