Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 3,488,817 C→T M130I (ATG→ATA)  glpR ← DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,488,8170CT100.0% 161.5 / NA 41M130I (ATG→ATA) glpRDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/23);  total (18/23)

TTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGC  >  REL606/3488745‑3488888
                                                                        |                                                                       
ttCGCCAATGATCCCGCTATCGCCGCTGCGTAATTCGCCNCCGGCAAAAATGATGNGGAAATTTTGTTTTACTATc                                                                      >  2:1333001/1‑76 (MQ=255)
ttCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATc                                                                      >  2:606100/1‑76 (MQ=255)
ttCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATc                                                                      >  2:440563/1‑76 (MQ=255)
ttCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATc                                                                      >  1:896895/1‑76 (MQ=255)
 tCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCa                                                                     <  2:573600/76‑1 (MQ=255)
 tCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCa                                                                     <  2:1278789/76‑1 (MQ=255)
   gCCAATGATCCCGCCGTCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAAc                                                                   <  1:1110898/76‑1 (MQ=255)
         gATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTa                                                             <  2:996077/76‑1 (MQ=255)
          aTCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAg                                                            <  1:452512/76‑1 (MQ=255)
           tCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGc                                                           <  1:388978/76‑1 (MQ=255)
             ccGCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGCaa                                                         >  2:740893/1‑76 (MQ=255)
              cGCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGCAAc                                                        <  1:852869/76‑1 (MQ=255)
               gCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACa                                                       >  2:402534/1‑76 (MQ=255)
                 cATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACAtt                                                     <  1:915600/76‑1 (MQ=255)
                      cgGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGAt                                                >  2:610094/1‑76 (MQ=255)
                          tGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGAttgtt                                            <  2:1165943/76‑1 (MQ=255)
                           gCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGAttgttg                                           <  1:1077486/76‑1 (MQ=255)
                           gCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGAttgttg                                           <  1:1005203/76‑1 (MQ=255)
                            cggaaTACGCCGCCGGCGAAAATCATGCGTGAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTgg                                          <  2:1214353/73‑1 (MQ=255)
                              tAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTATTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTg                                        >  1:1118203/1‑76 (MQ=255)
                               aaTTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGa                                       <  2:1126083/76‑1 (MQ=255)
                               aaTTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGa                                       <  2:819887/76‑1 (MQ=255)
                                   cGCCACCGGCGAAAATGATGTGGAAATCTTGTTTTGCTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAAt                                   <  2:1063721/76‑1 (MQ=255)
                                   cGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAAt                                   <  2:243709/76‑1 (MQ=255)
                                     ccACCGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATAc                                 >  1:730347/1‑76 (MQ=255)
                                        ccGGCGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCa                              <  1:781241/76‑1 (MQ=255)
                                            cGAAAATGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAAtt                          >  1:264464/1‑76 (MQ=255)
                                              aaaaTGCTGCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGc                        >  1:1254687/1‑76 (MQ=255)
                                              aaaaTGATGCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGc                        >  2:183596/1‑76 (MQ=255)
                                                 atgatgCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGCtgt                     <  2:896895/76‑1 (MQ=255)
                                                  tgatgCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGCtgtg                    >  2:882010/1‑76 (MQ=255)
                                                    atgCGGAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGCTGTGAt                  >  1:629230/1‑76 (MQ=255)
                                                        ggAAATCTTGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAag              <  2:1005876/76‑1 (MQ=255)
                                                           aaTCTTGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAagcag           <  2:68408/76‑1 (MQ=255)
                                                               ttGTTTTACTATCAACGTGTTAGCAACATTGAGTTTACTGGTGACAAGACGCAAATTGCTGTGATTAAGCAGcgcg       <  2:720606/76‑1 (MQ=255)
                                                                tGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGt      >  1:355647/1‑76 (MQ=255)
                                                                tGTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGt      >  2:1041425/1‑76 (MQ=255)
                                                                 gTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTg     <  2:1149844/76‑1 (MQ=255)
                                                                 gTTTTACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTg     <  2:931680/76‑1 (MQ=255)
                                                                    ttACTATCAACGTGTTTGCAACATTGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTgcgc  >  2:238247/1‑76 (MQ=255)
                                                                    ttACTATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGCTGTCATTAAGCAGCGCGTgcgc  >  2:1177871/1‑76 (MQ=255)
                                                                        |                                                                       
TTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAAAATGATGCGGAAATCTTGTTTTACCATCAACGTGTTAGCAACATTGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTAAGCAGCGCGTGCGC  >  REL606/3488745‑3488888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑