| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,162,569 | A→C | I572L (ATC→CTC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,162,569 | 0 | A | C | 97.5% | 155.3 / NA | 40 | I572L (ATC→CTC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (21/18); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCG > REL606/4162497‑4162641 | cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCa < 1:933942/76‑1 (MQ=255) gTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAActctc > 1:1065505/1‑76 (MQ=255) tACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAActctct < 2:559966/76‑1 (MQ=255) ccacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTg < 2:1198465/75‑1 (MQ=255) acacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTg > 2:1156246/1‑76 (MQ=255) cccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTcc < 1:1042326/76‑1 (MQ=255) gACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCgtg > 1:1209196/1‑76 (MQ=255) aCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCgtgt > 2:1274122/1‑76 (MQ=255) tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTAc < 1:867371/76‑1 (MQ=255) tACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGcac < 1:653321/76‑1 (MQ=255) aCGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGcaca < 2:626256/76‑1 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTa < 1:239990/76‑1 (MQ=255) cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTa > 1:261299/1‑76 (MQ=255) cgcgAATGTACAATCGAAACCGCTGAATGTCCTAGCCTTGGCCTGGTCATCTCTCTGAGCGTGTACGCACAGACTa < 1:1341115/76‑1 (MQ=255) gcgTATTTCAAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTNTANGCACAGACTaa > 2:210203/1‑76 (MQ=255) gcgTATGTCCAATCGAAACCTCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGGCTGTATACGCACAGACTaa > 1:60470/1‑76 (MQ=255) tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGa < 1:803933/76‑1 (MQ=255) aTGTCCAATCGAAAGCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACTGACTAACGaa < 1:115427/76‑1 (MQ=255) aTGTCCAATCGAAACCCCTGAAGGTCCGAACCTCNGTCTACTNTANTCNCTGTACGTGTACGCACAGACTAACGaa > 1:1163535/1‑76 (MQ=255) aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGaa > 1:836970/1‑76 (MQ=255) ccAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACg > 2:173867/1‑76 (MQ=255) cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg > 2:1058401/1‑76 (MQ=255) aaTCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc > 1:532164/1‑76 (MQ=255) aTCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCt < 2:250849/76‑1 (MQ=255) aTCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTACGTGTACGCACAGACTAACGAATACGGCt > 2:33173/1‑76 (MQ=255) tCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt < 2:539259/76‑1 (MQ=255) aaaCCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt < 2:173018/76‑1 (MQ=255) aaCCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt > 2:122802/1‑76 (MQ=255) ccTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgaga > 2:513226/1‑76 (MQ=255) ccTGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgaga > 2:320146/1‑76 (MQ=255) tGAAGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTTAGACt > 1:598800/1‑76 (MQ=255) aGGTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCg < 1:1137040/76‑1 (MQ=255) gTCCGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTGACTAATACGGCTTCCTTGAGACTCCGTa > 1:214856/1‑76 (MQ=255) cGAACATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTNTCg < 1:1207304/76‑1 (MQ=255) aaCATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa > 1:45482/1‑76 (MQ=255) aaCATCGGTCNGATCAACTCTNTGTCCGTGTACGAACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa > 2:1323172/1‑76 (MQ=255) aCATCGGTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaa < 1:1063686/76‑1 (MQ=255) cGGTCTGCTCAACTCTCTGTCCGTGNATGCACAGACGAACGAATACANCTTGCTTGAGACTCCGTATCGTAAAGTg < 1:194674/76‑1 (MQ=255) ggTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGa > 2:602558/1‑76 (MQ=255) ggTCTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGa > 2:706774/1‑76 (MQ=255) cTGCTCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg < 2:732735/76‑1 (MQ=255) | CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCG > REL606/4162497‑4162641 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |