Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,080,405	A→G	Y27C (TAC→TGC)	yccE →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,080,405	0	A	G	100.0%	233.6 / NA	54	Y27C (TAC→TGC)	yccE	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/30);  total (24/30)

AATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATG  >  REL606/1080335‑1080476                                                                       |                                                                        aaTATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGaaa                                                                    <  1:441982/76‑1 (MQ=255)   tatcCATGGAACTAGTTGCACTGCAAATAATTAGTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGaaaaa                                                                  >  1:1225973/1‑76 (MQ=255)    ataCATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGaaaaaa                                                                 <  2:946091/76‑1 (MQ=255)        aTGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCa                                                             <  1:831982/76‑1 (MQ=255)          ggAATTAGTTGCACTGCAAATAATTNTCTGAAACAGGCCTGGAACGANATAGAAAATGAGTGCGAAAAAAATCaaa                                                           <  1:196481/76‑1 (MQ=255)          ggAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCaaa                                                           <  1:728163/76‑1 (MQ=255)          ggAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCaaa                                                           <  1:488905/76‑1 (MQ=255)           gAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAANAATCAAAc                                                          <  1:1289856/76‑1 (MQ=255)           gAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAAc                                                          >  1:1182691/1‑76 (MQ=255)            aaTTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACa                                                         <  2:1024140/76‑1 (MQ=255)            aaTTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATAAAACa                                                         >  2:47797/1‑76 (MQ=255)             aTTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACat                                                        <  2:991907/76‑1 (MQ=255)              ttAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACata                                                       >  1:120286/1‑76 (MQ=255)              ttAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACata                                                       <  1:1139040/76‑1 (MQ=255)               tAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACatat                                                      <  2:571102/76‑1 (MQ=255)                aGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATAtt                                                     >  2:1105963/1‑76 (MQ=255)                aGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATAtt                                                     <  2:418718/76‑1 (MQ=255)                    gCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAAt                                                 <  2:1251476/76‑1 (MQ=255)                    gCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAAt                                                 <  1:93745/76‑1 (MQ=255)                     cACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATc                                                <  1:898647/76‑1 (MQ=255)                     cACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATc                                                <  1:898639/76‑1 (MQ=255)                       cTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCAc                                              <  2:288276/76‑1 (MQ=255)                       cTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCG‑AAAAAATCAAACATATTCAATCACg                                             >  1:1243216/1‑76 (MQ=255)                        tGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACg                                             >  1:707733/1‑76 (MQ=255)                        tGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACg                                             <  1:1107507/76‑1 (MQ=255)                         gCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGc                                            >  2:62047/1‑76 (MQ=255)                           aaaTAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCtt                                          >  2:1252678/1‑76 (MQ=255)                                  tattTGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGaaa                                   >  2:1102940/1‑76 (MQ=255)                                   attTGAAACAGGCCTGGAAAGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGaaaa                                  >  1:426112/1‑76 (MQ=255)                                     ttGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAaca                                <  2:501574/76‑1 (MQ=255)                                     ttGAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAaca                                >  2:175000/1‑76 (MQ=255)                                       gAAACAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAacaca                              >  1:426897/1‑76 (MQ=255)                                        aaaCAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAacacac                             >  2:1195145/1‑76 (MQ=255)                                         aaCAGGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACt                            >  1:1003332/1‑76 (MQ=255)                                         aaCACGCCTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACt                            >  2:189054/1‑76 (MQ=255)                                              gCCTGGAACGATAT‑AAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtgtg                      <  2:839510/76‑1 (MQ=255)                                               ccTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtgtg                      <  2:839520/76‑1 (MQ=255)                                               ccTGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtgtg                      >  1:340115/1‑76 (MQ=255)                                                 tGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGtt                    >  2:414882/1‑76 (MQ=255)                                                 tGGAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGtt                    >  1:708575/1‑76 (MQ=255)                                                  ggAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGttt                   <  2:1260596/76‑1 (MQ=255)                                                  ggAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGttt                   <  2:865941/76‑1 (MQ=255)                                                   gAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGtttt                  <  1:308759/76‑1 (MQ=255)                                                   gAACGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGtttt                  <  1:308767/76‑1 (MQ=255)                                                      cGATATAAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATg               <  2:571244/76‑1 (MQ=255)                                                         tataAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCgg            <  1:832359/76‑1 (MQ=255)                                                          ataAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGt           >  2:390737/1‑76 (MQ=255)                                                           taAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGtt          >  2:1261359/1‑76 (MQ=255)                                                           taAAAAATGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGtt          >  2:1261350/1‑76 (MQ=255)                                                             aaaaaTGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTtat        <  2:476517/76‑1 (MQ=255)                                                               aaaTGAGTGCGAAAAACATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTGATACGGTTATAc      >  1:8855/1‑76 (MQ=255)                                                               aaaTGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATAc      <  1:481773/76‑1 (MQ=255)                                                                  tGAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAAt   >  1:906117/1‑76 (MQ=255)                                                                   gAGTGCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATg  <  1:387667/76‑1 (MQ=255)                                                                       |                                                                        AATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATG  >  REL606/1080335‑1080476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑