| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,658,770 | T→G | N200K (AAT→AAG) | ydgB → | short chain dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,658,770 | 0 | T | G | 95.6% | 170.1 / ‑4.3 | 46 | N200K (AAT→AAG) | ydgB | short chain dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (26/17); total (27/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGC > REL606/1658700‑1658842 | aTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATc < 2:606750/76‑1 (MQ=255) ttCTATTGCGCCATNGCTGATCCTGTTTAATGAACATGATGATGCCGTATATCGACAACAGGCGCTTAATNACTCa < 2:1343665/76‑1 (MQ=255) ttCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCa > 1:620491/1‑76 (MQ=255) ttCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCa > 1:121363/1‑76 (MQ=255) ttCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATACCGACAACAGGCGCTGAATAAATCa > 1:695296/1‑76 (MQ=255) tCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCAc < 1:256506/76‑1 (MQ=255) tATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACtg > 2:1198224/1‑76 (MQ=255) tATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACtg > 2:1198236/1‑76 (MQ=255) aTTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACtga > 2:285565/1‑76 (MQ=255) aTTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACtga < 1:385497/76‑1 (MQ=255) ttGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACtgat > 1:1219810/1‑76 (MQ=255) tGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACtgatg > 2:179161/1‑76 (MQ=255) gcCATCGCTGATCCNGTTTAATGAACATGATGATGCCGAATACCGACAACAGGCGCTGAACAACTCACTGATGaaa < 1:1144814/76‑1 (MQ=255) gcCATCGCTGATACTCTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGNAATCACTGATGaaa > 1:1151914/1‑76 (MQ=255) tCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACcg > 2:880726/1‑76 (MQ=255) gCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACcgcg < 2:988241/76‑1 (MQ=255) gCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACcgcg < 1:770030/76‑1 (MQ=255) cTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCgcgc > 2:576015/1‑76 (MQ=255) gATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCt < 1:830384/76‑1 (MQ=255) aTCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTg > 1:1222162/1‑76 (MQ=255) tCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTgg > 1:756500/1‑76 (MQ=255) cTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCg < 2:103102/76‑1 (MQ=255) tGTTTAATGAATATGATGATGCCGAATATCGACAACAGGTGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCga > 1:36704/1‑76 (MQ=255) tttAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCgaga > 2:579533/1‑76 (MQ=255) ttAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGagaa < 1:701537/76‑1 (MQ=255) tGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGagaaagaa < 1:452629/76‑1 (MQ=255) tGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGagaaagaa > 2:1102386/1‑76 (MQ=255) gatgatgCCGAATATCGACAACAGGCGCTGAAGGAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCg > 1:154917/1‑76 (MQ=255) gatgatgCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCg > 1:1084082/1‑76 (MQ=255) tgatgCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGAc > 2:779790/1‑76 (MQ=255) gCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTgg > 2:967570/1‑76 (MQ=255) ccGAATATCGACAACACGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGt < 1:398831/76‑1 (MQ=255) cGAATATCGACAACAGGCGCTGAAGAAATCCCTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTc < 2:617279/76‑1 (MQ=255) cGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTc > 2:808939/1‑76 (MQ=255) tatCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAtt > 2:897677/1‑76 (MQ=255) taccGACAACAGGCGCTGAAGAAATCACTGATGAAACCCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAtt < 1:435594/73‑1 (MQ=255) atCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAtta > 2:1273472/1‑76 (MQ=255) tCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttac > 1:550633/1‑76 (MQ=255) tCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttac < 1:256638/76‑1 (MQ=255) tCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttac < 2:1000501/76‑1 (MQ=255) caacaGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttac > 2:362527/1‑76 (MQ=255) aacaGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttacn < 1:837668/76‑2 (MQ=255) aGGCGCTGAAGAAATCACTTATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttacttac < 2:395670/76‑1 (MQ=255) cgcTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAg > 1:357613/1‑76 (MQ=255) cTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTTGATGACTTACTTACCAGtt < 2:117411/76‑1 (MQ=255) cTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGtt < 2:178802/76‑1 (MQ=255) cTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGtt > 1:795619/1‑76 (MQ=255) tGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTg < 1:520467/76‑1 (MQ=255) gaagaaATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGc < 2:304628/76‑1 (MQ=255) | ATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGC > REL606/1658700‑1658842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |