Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	3,755,753	(GTC)3→2	coding (722‑724/864 nt)	yicC →	hypothetical protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	3755750 =	1 (0.030)	33 (0.910)	27/138	0.4	97.2%	coding (719/864 nt)	yicC	hypothetical protein
	?	REL606	= 3755746	1 (0.030)					coding (715/864 nt)	yicC	hypothetical protein

ACTTCGGCATTGATAGATTTCGACGCAAGAGTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3755825‑3755750 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gacgacCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGACATGCGCTTCCAGGCGGTCCAGTTCTTCGG  <  REL606/3755746‑3755675                                                                                                                                                      ACTTCGGCATTGATAGATTTCGACGCAAGAGTGTTCGTCTCGCGGTTGAATTCCTGCATCATAAGGTCCAGACGAC                                                                          <  1:65431/76‑1  CTTCGGCATTGATAGATTTCGACGCAAGAGTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACC                                                                         <  2:1116634/76‑1           TGATAGATTTCGACGCAAGAGTGTTCGACTCGCTGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTC                                                                >  1:762881/1‑76           TGATAGATTTCGACGCAAGAGTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTC                                                                >  2:934132/1‑76           TGATAGATTTCGACGCAAGAGTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTC                                                                <  2:415943/76‑1             ATAGATTTCGACGCAAGAGTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTT                                                              <  1:506901/76‑1             ATAGATTTCGACGCAAGAGTGTTCCACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTT                                                              >  1:961785/1‑76              TAGATTTCGACGCAAGAGTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTT                                                             <  2:69506/76‑1               AGATTTCGNCGCGAGAGTGTTCGAGTCTCGGTTGGATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTT                                                            <  2:201216/76‑1                       ACGCAAGAGTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAG                                                    <  2:817841/76‑1                         GCAAGAGTGTTCGACTCGAGGTGGAATNCCNGCNTCTTAAANTCTAGATGACCAACGGCTTCTTTTTTCTTCAGAA                                                  <  1:1101566/76‑1                         GCAAGAGTGTTCGACCCGCGGTTGAATTCCTGCCTCATAAAGTCCAGACGACCAACGGCTTNTTTTTTCTTCAGAA                                                  >  1:1093194/1‑76                          CAAGAGTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAAT                                                 >  2:990322/1‑76                          CAAGAGTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAAT                                                 <  2:665225/76‑1                          CAAGAGTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAAT                                                 <  1:395494/76‑1                            AGAGTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTTAGAATGT                                               >  2:978105/1‑76                            AGAGTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGT                                               >  1:770163/1‑76                              AGTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTG                                             >  1:137871/1‑76                              AGTGTTCGACTCGCCGTTGAATTGCTTCATCATAAAGTCCAGACGACCTACGGCTTCTTTTTTCTTCAGAATGTTG                                             <  2:14662/76‑1                               GTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGNATGTTGT                                            <  2:1024726/76‑1                               GTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGT                                            <  2:1063389/76‑1                               GTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGT                                            <  2:1063409/76‑1                                    CGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTC                                       <  2:242975/76‑1                                    CGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTC                                       <  2:338229/76‑1                                     GACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTTTAGGTCT                                      >  1:257575/1‑76                                       CTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCT                                    <  2:766258/76‑1                                          GCGGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTG                                 <  1:547106/76‑1                                            GGTTGAATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGAC                               <  1:217052/76‑1                                                  ATTCCTGCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGACATGCGC                         <  1:626244/76‑1                                                        GCATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGACATGCGCTTCCAG                   <  2:59793/76‑1                                                          ATCATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGACATGCGCTTCCAGGC                 <  2:527843/76‑1                                                             ATAAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGACATGCGCTTCCAGGCGGT              >  1:680333/1‑76                                                               AAAGTCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGACATGCGCTTCCAGGCGGTCC            >  1:364711/1‑76                                                                   TCCAGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGACATGCGCTTCCAGGCGGGCCAGTT        >  2:381789/1‑76                                                                         CGACCAACGGCTTCTTTTTTCTTTAGAATGTTGTAGGTCTCTTTGACATGCGCTTCCAGGCGGTCCAGTTCTTCGG  >  1:994560/1‑76                                                                         CGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGACATGCGCTTCCAGGCGGTCCAGTTCTTCGG  <  1:171810/76‑1                                                                         CGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGACATGCGCTTCCAGGCGGTCCAGTTCTTCGG  >  1:423603/1‑76                                                                                                                                                      ACTTCGGCATTGATAGATTTCGACGCAAGAGTGTTCGACTCGCGGTTGAATTCCTGCATCATAAAGTCCAGACGAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/3755825‑3755750 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gacgacCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGACATGCGCTTCCAGGCGGTCCAGTTCTTCGG  <  REL606/3755746‑3755675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.