| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 473,924 | A→C | V461G (GTC→GGC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 473,924 | 0 | A | C | 97.2% | 131.2 / ‑4.5 | 36 | V461G (GTC→GGC) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (23/12); total (24/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
CAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGA > REL606/473851‑473996 | cAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGccc > 2:457664/1‑76 (MQ=255)cAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGccc > 2:702477/1‑76 (MQ=255) gCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCcccc > 2:722795/1‑76 (MQ=255) cATTTTGCACATACCAGATGCGCCAGTTGAATAGTTTCTTCGTAAGCCCGATTGCCCAATACTGCGCGGCCCAcgc > 1:1225495/1‑76 (MQ=255) tttGCACACTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGct > 1:1174262/1‑76 (MQ=255) tGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGctct < 2:236042/76‑1 (MQ=255) gCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGAGGCCCCCGCGCtctc > 1:1045912/1‑76 (MQ=255) caTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCa > 2:59353/1‑76 (MQ=255) caTTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCa < 1:678336/76‑1 (MQ=255) ttCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGc > 2:823870/1‑76 (MQ=255) aGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcat < 2:381678/76‑1 (MQ=255) gATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatc < 1:209711/76‑1 (MQ=255) aTGCGCCAGTTGCATAATTTCTTCGTTCGCCCCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatca > 2:530315/1‑76 (MQ=255) cgcCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAaca > 1:360308/1‑76 (MQ=255) aGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACggg > 2:1178127/1‑76 (MQ=255) ttGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCACCACGGGtt > 1:91308/1‑76 (MQ=255) tGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTc > 2:1125863/1‑76 (MQ=255) cATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGt > 2:748613/1‑76 (MQ=255) aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg > 1:47447/1‑76 (MQ=255) tttCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCCACACCGGGTCGTGACGt > 1:411667/1‑76 (MQ=255) tttCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGt > 1:680510/1‑76 (MQ=255) ttcttgGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtc > 1:77039/1‑76 (MQ=255) tcttcGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtct < 1:747692/76‑1 (MQ=255) cttcGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtctc > 2:249140/1‑76 (MQ=255) ttcgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCa < 1:463084/76‑1 (MQ=255) gttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATc > 2:947129/1‑76 (MQ=255) ccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcaccac < 2:669119/76‑1 (MQ=255) gcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCAccacca < 1:731354/76‑1 (MQ=255) cATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAg > 2:142304/1‑76 (MQ=255) aTTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGc > 2:1065670/1‑76 (MQ=255) aTTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGc < 1:421983/76‑1 (MQ=255) aTTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGc > 2:794279/1‑76 (MQ=255) aTTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGc > 2:887431/1‑76 (MQ=255) tGCCCAATACTGCGNGGCCCCCGCTCTCTCGCAGCTCGTCAACACGGGTTCGTGACGTCTCAATCACCACCAGCgg > 1:1144/1‑76 (MQ=255) tGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCgg > 2:441456/1‑76 (MQ=255) gCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGa < 2:503436/76‑1 (MQ=255) cAATACTGCGCCGCCCCCGCGCTCTCGCAGCTCCTCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAata > 1:1165095/1‑76 (MQ=255) aTACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATc > 1:759071/1‑76 (MQ=255) aCTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCag < 1:169829/76‑1 (MQ=255) gcgcGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAgg < 2:722020/76‑1 (MQ=255) cgGCCCCCGCGCTCTCGCAGCTCATCAACTCGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGGGa > 2:636382/1‑76 (MQ=255) | CAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGA > REL606/473851‑473996 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |