| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,928,260 | T→A | I15N (ATC→AAC) | rho → | transcription termination factor Rho |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,928,260 | 0 | T | A | 97.2% | 128.4 / ‑3.0 | 36 | I15N (ATC→AAC) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (18/17); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01 | |||||||||||
TTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCT > REL606/3928188‑3928332 | ttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAacac > 2:1140584/1‑76 (MQ=255) tACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACAct > 2:975496/1‑76 (MQ=255) cccAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGNACACtctc < 1:1210568/76‑1 (MQ=255) cAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCNGGTTGCTCAGCTGAACACTCGCgg < 1:106282/76‑1 (MQ=255) cAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCgg < 1:888588/76‑1 (MQ=255) aaGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGc > 2:633333/1‑76 (MQ=255) aGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACGCTCGGCg < 2:487396/76‑1 (MQ=255) aGAACTCATACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTTAACACTCTCGGCGAAAata > 2:18446/1‑76 (MQ=255) aGAACTCACACCACTATGAATCTTACCGACTTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAata < 2:34249/76‑1 (MQ=255) cTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATggg > 1:559430/1‑76 (MQ=255) tCACACCACTATGANTCTTATCGAACTAAAGAATCCGCCAGTTCCTGAGCAGAACACTCTCGGCGAATATATgggg < 2:28161/76‑1 (MQ=255) acacCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCt < 1:985922/76‑1 (MQ=255) accaccCTGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTgg > 1:1188431/1‑76 (MQ=255) tATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGANAAc < 1:1140585/76‑1 (MQ=255) tATGAATCTTAACGAATTAAAGAATACGCCGGTCTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAAc > 1:1163031/1‑76 (MQ=255) aTGAATCTTTCCGAATTAAAGAATACGCCGGNGTCTAAGGTGAACACTCTCGGCGAAAATATGCNGCTGGAAAAcc > 1:149979/1‑76 (MQ=255) gAATCTTACCGAATCAAAGAATACGCCGGTGTCTGAGCTCAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTg < 1:18446/76‑1 (MQ=255) tCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCt < 2:761946/76‑1 (MQ=255) cTTACCGAATTAGAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTc < 1:1017059/76‑1 (MQ=255) gAATTAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGc < 1:855734/76‑1 (MQ=255) tAAAGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTaa > 1:572297/1‑76 (MQ=255) aaaGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAg < 1:344258/76‑1 (MQ=255) aaGAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGc < 2:567187/76‑1 (MQ=255) gAATACGCCGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAg < 1:557982/76‑1 (MQ=255) ccGGTTCCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACatta > 1:1185167/1‑76 (MQ=255) cGGTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACattat > 2:516268/1‑76 (MQ=255) ggTTTCTGAGCTGAACACTCTCGGCGAAAATGTGNGGCTTGANCANCTNGCTCTCATGCGCAAGCAGGACattatt > 1:1105333/1‑76 (MQ=255) gTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTAttt < 2:501440/76‑1 (MQ=255) gTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTAttt < 2:147370/76‑1 (MQ=255) gTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTAttt > 2:574898/1‑76 (MQ=255) gTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTAttt > 1:501140/1‑76 (MQ=255) gTTTCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTAttt > 2:911905/1‑76 (MQ=255) tttCTGAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTAtttt > 2:939817/1‑76 (MQ=255) gAGCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCa > 2:930786/1‑76 (MQ=255) gCTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATc < 2:1203839/76‑1 (MQ=255) cTGAACACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATcc > 1:1024082/1‑76 (MQ=255) tGAACACTCTCGTCGAAAAAATGGGGCTGAAAATCCTGACTCGACTGCGTAAGCAGGACATTATTTTATCCATCCt > 1:1222655/1‑76 (MQ=255) | TTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCT > REL606/3928188‑3928332 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |