| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,404,055 | T→A | I21N (ATC→AAC) | rpsR → | 30S ribosomal protein S18 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,404,055 | 0 | T | A | 97.5% | 150.2 / ‑5.8 | 41 | I21N (ATC→AAC) | rpsR | 30S ribosomal protein S18 |
| Reads supporting (aligned to +/- strand): ref base T (1/0); major base A (17/22); minor base G (1/0); total (19/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
AGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTATC > REL606/4403983‑4404125 | agaCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGa > 1:784390/1‑76 (MQ=255) aCTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACt < 1:663140/76‑1 (MQ=255) tAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACtat > 1:593450/1‑76 (MQ=255) aGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACtata > 2:669954/1‑76 (MQ=255) gCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCNGTGTCACCGCGGAAGGCGTTCAAGAGAACGACTATaa < 1:1233256/76‑1 (MQ=255) cATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAg < 1:1200489/76‑1 (MQ=255) cATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAg < 2:358433/76‑1 (MQ=255) gCACGTTATTTCCGTCGTCCCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCg > 1:884443/1‑76 (MQ=255) tttCCTTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTAGGCTg < 2:149191/76‑1 (MQ=255) tttCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTg > 1:972085/1‑76 (MQ=255) cgtcgtCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGaaaa < 1:120086/76‑1 (MQ=255) gtcgCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTa > 2:525025/1‑76 (MQ=255) gtcgCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTa > 2:37831/1‑76 (MQ=255) gCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACAt < 1:239950/76‑1 (MQ=255) cAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATc < 1:745018/76‑1 (MQ=255) cAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATc < 1:673715/76‑1 (MQ=255) aaGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCa < 1:659149/76‑1 (MQ=255) aGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAGAACTACATCAc < 1:35035/76‑1 (MQ=255) tCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGa > 1:969668/1‑76 (MQ=255) tGCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGaaa < 1:364173/76‑1 (MQ=255) gCCGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATAGCGCTACGCTGAAAAACTACATCACCGAAAg > 1:27210/1‑76 (MQ=255) cGTTTCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCg > 1:357274/1‑76 (MQ=255) tttCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGt < 1:543935/76‑1 (MQ=255) ttCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCNGTa < 1:1142204/76‑1 (MQ=255) ttCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTa > 1:592268/1‑76 (MQ=255) ttCACCGCGGAAGGCGTTAAAGGGAACNACNACNAACATATNGCGACGATGAAAAACTACATCACCGAAAGCGGTa < 1:1101773/76‑1 (MQ=255) tCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTaa > 1:115345/1‑76 (MQ=255) tCACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTaa > 2:31728/1‑76 (MQ=255) cACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAg < 1:261654/76‑1 (MQ=255) cACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAg > 2:419224/1‑76 (MQ=255) cACCGCGGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAg > 1:388792/1‑76 (MQ=255) cgcgGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGAtt < 2:507097/76‑1 (MQ=255) gcgGAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTg < 1:699241/76‑1 (MQ=255) gcgGAAGGCGTCCCAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGAttc < 2:16150/76‑2 (MQ=255) cgGAAGGCGTTCAAGAGAGCGNCTNTAAAGATTTCGCTACGCTGAANAACTACANCNCCGAAAGCGGTAAGATTGt > 2:182360/1‑76 (MQ=255) gAAGGCGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTcc > 2:489048/1‑76 (MQ=255) cGTTCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGc < 1:837808/76‑1 (MQ=255) ttCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCg < 1:231624/76‑1 (MQ=255) ttCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCg < 2:511197/76‑1 (MQ=255) tCAAGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGt > 1:833141/1‑76 (MQ=255) aaGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTAt < 1:514330/76‑1 (MQ=255) aaGAGAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTAt > 1:525475/1‑76 (MQ=255) agagAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTATc < 2:520111/76‑1 (MQ=255) agagAACGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTATc < 2:563630/76‑1 (MQ=255) | AGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTACATCACCGAAAGCGGTAAGATTGTCCCAAGCCGTATC > REL606/4403983‑4404125 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |