Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,304,967	G→A	R425W (CGG→TGG)	cls ←	cardiolipin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,304,967	0	G	A	93.8%	104.9 / ‑1.4	32	R425W (CGG→TGG)	cls	cardiolipin synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (11/19);  total (11/21)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

GCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCGACCAGCACGCTCTTGGTATGCAGTAACC  >  REL606/1304897‑1305038                                                                       |                                                                        gcggcAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCACatat                                                                    <  2:638567/76‑1 (MQ=255)  cggcAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCACATATc                                                                   <  1:465954/76‑1 (MQ=255)     cAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCACATATCAAg                                                                <  2:671299/76‑1 (MQ=255)     cAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCACATATCAAg                                                                >  2:542332/1‑76 (MQ=255)     cAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCACATATCAAg                                                                <  2:379561/76‑1 (MQ=255)      aaGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCACATATCAAgg                                                               >  1:422682/1‑76 (MQ=255)        ggTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCACATATCAAGGtt                                                             >  1:749553/1‑76 (MQ=255)          tCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCACATATCAAGGTTaa                                                           <  1:1014193/76‑1 (MQ=255)           cTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCACATATCAAGCTGAAc                                                          <  1:1172615/76‑1 (MQ=255)              cACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAGGTTAACGGt                                                       <  1:503849/76‑1 (MQ=255)                   aaaCCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCACATATCAAGGTTAACGGCGCCaa                                                  >  2:1154902/1‑76 (MQ=255)                    aaCCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCACATATCAAGGTTAACGGTGCCAAc                                                 <  1:755014/76‑1 (MQ=255)                      ccTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCACATATCAAGGTTAACGGTGCCAACCa                                               >  1:783578/1‑76 (MQ=255)                        tttATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCACATATCAAGGTTAACGGTGCCAACCAGa                                             >  1:456450/1‑76 (MQ=255)                                 gATTGCCAGAGTAATCTCGAAATATAGNCACAGACTCCACATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCa                                    >  2:4763/1‑76 (MQ=255)                                      ccAGAGTACTCTCGAAATTTAGCCACAGACTCGACTGATCAAGGCTAACGGTGCGACCCAGGCTTAGTTCACCATc                               >  2:18918/1‑76 (MQ=255)                                      ccAGAGTAATCTCGAAATTTAGCCACAGACTCCACATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATc                               <  1:555072/76‑1 (MQ=255)                                       cAGAGTAATCTCGAAATTTAGCCACAGACTCCACATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCg                              <  2:606745/76‑1 (MQ=255)                                       cAGAGTAATCTCGAAATTTAGCCACAGACTCCACATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCg                              <  1:340786/76‑1 (MQ=255)                                        agagTAATCTCGAAATTTAGCCACAGACTCCACATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCGa                             <  1:453572/76‑1 (MQ=255)                                          agTAATCTCGAAATTTAGCCACAGACTCCACATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCGAcc                           <  2:251562/76‑1 (MQ=255)                                            tAATCTCGAAATTTAGCCACAGACTCCACATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCGACCAg                         <  1:314298/76‑1 (MQ=255)                                             aaTCTCGAAATTTAGCCACAGACTCCACATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCGACCAGc                        <  1:596820/76‑1 (MQ=255)                                               tctcGAAATTTAGCCACAGACTCCACATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCGACCAGCAc                      <  1:731906/76‑1 (MQ=255)                                                     aaTTTAGCNACACACTCCGCATATGAACGTTAATGTTGCCAACCAGACTTAGTTCACCATCGACCAGCACGCTCtt                <  2:203131/76‑1 (MQ=255)                                                     aaTTTAGCCACAGACTCCACATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCGACCAGCACGCTCtt                >  2:845773/1‑76 (MQ=255)                                                       tttAGCCACAGACTCCACGTATTAAGGNTANCGNTGTCAACNAGGCTTCGTTCACCATCGACCAGCACGCTCTTgg              <  1:1099506/76‑1 (MQ=255)                                                       tttAGCCACAGACTCCACATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCGACCAGCACGCTCTTgg              >  2:302217/1‑76 (MQ=255)                                                             cacaGACTCCACATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCGACCAGCACGCTCTTGGTATGCa        <  2:257735/76‑1 (MQ=255)                                                                aGACTCCACATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCGACCAGCACGCTCTTGGTATGCAGTa     <  1:131475/76‑1 (MQ=255)                                                                  aCTCCACATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCGACCAGCACGCTCTTGGTATGCAGTAAc   >  2:38209/1‑76 (MQ=255)                                                                   cTCCACATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCGACCAGCACGCTCTTGGTATGCAGTAAcc  <  2:566832/76‑1 (MQ=255)                                                                       |                                                                        GCGGCAAGGTCTGCACCAAAACCTTTATCGTCGATTGCCAGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCGACCAGCACGCTCTTGGTATGCAGTAACC  >  REL606/1304897‑1305038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑