Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,579,770	C→T	M175I (ATG→ATA)	yneH ←	predicted glutaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,579,770	0	C	T	100.0%	89.9 / NA	25	M175I (ATG→ATA)	yneH	predicted glutaminase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/12);  total (13/12)

AGCGCAGTAATGAAAGTAGTTTTGCAGAACGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTCATCAGCCAGGCGATAGCCGCATTTCGCGAGGAATGTTCAAATTCGGAACGCGCTACCACCGTATCGTAGG  >  REL606/1579704‑1579840                                                                   |                                                                       agCGCAGTAATGAAAGTAGTTTTGCAGAACGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAg                                                               >  2:337120/1‑76 (MQ=255)  gcgcAGTAATGAAAGTAGTTTTGCAGAACGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAgn                                                              <  1:258282/76‑2 (MQ=255)      aGTAATGAAAGTAGTTTTGCAGAACGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGAt                                                          <  1:999129/76‑1 (MQ=255)            naaaGTAGTTTTGCAGAACGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGc                                                    >  1:309453/2‑76 (MQ=255)             aaaGTAGTTTTGCAGAACGGTTGTCACGTCATGATGGAAATTGCCACACGACTTTATCAGCCAGGCGATAGCCGCa                                                   >  2:40512/1‑76 (MQ=255)             aaaGTAGTTTTGCAGAACGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCa                                                   >  2:1075385/1‑76 (MQ=255)               agtagtTTTGCAGAACGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCAtt                                                 >  2:727434/1‑76 (MQ=255)                 tagtTTTGCAGAACGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCATTTc                                               >  2:797217/1‑76 (MQ=255)                 tagtTTTGCAGAACGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCATTTc                                               >  2:797209/1‑76 (MQ=255)                    ttttGCAGTACGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCATTTcgcg                                            <  1:829911/76‑1 (MQ=255)                         cAGAACGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCATTTCGCGAGGaa                                       >  2:377662/1‑76 (MQ=255)                         cAGAACGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGAGAGAGCCGCATTTCGCGAGGaa                                       >  1:60091/1‑76 (MQ=255)                          aGAACGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCATTTCGCGAGGAAt                                      <  2:1172524/76‑1 (MQ=255)                           gAACGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCATTTCGCGAGGAATg                                     <  2:387351/76‑1 (MQ=255)                            aaCGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCATTTCGCGAGGAATGt                                    >  1:1002359/1‑76 (MQ=255)                            aaCGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCATTTCGCGAGGAATGt                                    <  2:804646/76‑1 (MQ=255)                               ggTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCATTTCGCGAGGAATGTTCa                                 >  1:467962/1‑76 (MQ=255)                                gTTGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCATTTCGCGAGGAATGTTCaa                                <  1:750033/76‑1 (MQ=255)                                  tGTCACGTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCATTTCGCGAGGAATGTTCAAAt                              <  1:1145922/76‑1 (MQ=255)                                        gTCATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCATTTCGCGAGGAAAGTTCAAATTCGGaa                        <  1:111203/76‑1 (MQ=255)                                          cATGATGGAAATTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCATTTCGCGAGGAATGTTCAAATTCGGAAcg                      <  2:949802/76‑1 (MQ=255)                                                   aaTTGCCAAACGACTTTATCAGCCAGGCGATAGCCGCATTTCGCGAGGAATGTTCAAATTCGGAACGCGCTaccac             >  1:359224/1‑76 (MQ=255)                                                        ccAAACGACTTTATCAGCCAGGCGATAGCCGCATTTCGNGAGGAATGTTCAAATTCGGAACGCGCTACCACCGTAt        <  1:1238580/76‑1 (MQ=255)                                                        ccAAACGACTTTATCAGCCAGGCGATAGCCGCATTTCGCGAGGAATGTTCAAATTCGGAACGCGCTACCACCGTAt        <  2:741721/76‑1 (MQ=255)                                                        ccAAACGACTTNATNAGCCAGGCGATAGCCGCATTTCGCGAGGAATGTTCAAATTCGGGACGCGCTACCTCCGTAt        <  2:196584/76‑1 (MQ=255)                                                           aaCTACTTTATCAGCCAGGCGATAGCCGCATTTCGCGAGGAATGTTCAAATTCGGAACGCGCTACCACCGTATCGt     <  2:274740/76‑1 (MQ=255)                                                              gACTTTATCAGCCAGGCGATAGCCGCATTTCGCGAGGAATGTTCAAATTCGGAACGCGCTACCACCGTATCGTAgg  >  1:692869/1‑76 (MQ=255)                                                                   |                                                                       AGCGCAGTAATGAAAGTAGTTTTGCAGAACGGTTGTCACGTCATGATGGAAATTGCCAAACGACTTCATCAGCCAGGCGATAGCCGCATTTCGCGAGGAATGTTCAAATTCGGAACGCGCTACCACCGTATCGTAGG  >  REL606/1579704‑1579840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑