BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,658,199	T→G	L10* (TTA→TGA)	ydgB →	short chain dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,658,199	0	T	G	100.0%	204.3 / NA	50	L10* (TTA→TGA)	ydgB	short chain dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (24/26); total (24/26)

GTGGGATAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGAT  >  REL606/1658126‑1658271

gtggGATAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATatgaa                                                                        >  1:797781/1‑76 (MQ=255)

 tggGATAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATatgaat                                                                       >  2:928088/1‑76 (MQ=255)

  gggATAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATatgaatt                                                                      >  2:1156235/1‑76 (MQ=255)

     aTAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACt                                                                   >  1:326313/1‑76 (MQ=255)

     aTAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACt                                                                   <  2:612229/76‑1 (MQ=255)

      tAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTg                                                                  >  1:776777/1‑76 (MQ=255)

       aaCGTTCATCCCATCGTTGGATTGCNAAGGAGATAACGATGGGTAACNCCCCGCCCTTGCCAATATGAATTACTgg                                                                 <  1:183228/76‑1 (MQ=255)

          gTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCgg                                                              >  2:801586/1‑76 (MQ=255)

               tCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGgtc                                                         <  2:749781/76‑1 (MQ=255)

                 ccATCGTTGGATAGCAATCGAGATGAGGACAGGTGGAACACGGACCTTACTAATATGCATTAGTGGCGCAGctcgt                                                       >  1:1264929/1‑76 (MQ=255)

                  cATCGTTGNATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGgtggtc                                                      >  1:1220205/1‑76 (MQ=255)

                  cATCGTTGGATAGCAAAGGCGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGggcgtc                                                      >  1:1080149/1‑76 (MQ=255)

                    tCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGc                                                    <  2:419714/76‑1 (MQ=255)

                     cGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCa                                                   <  2:228060/76‑1 (MQ=255)

                     cGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCa                                                   <  2:1201971/76‑1 (MQ=255)

                      gTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCAt                                                  <  2:702427/76‑1 (MQ=255)

                       ttGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATCTGAATTACTGGCGGAGGTCGTCGCATc                                                 <  1:1125023/76‑1 (MQ=255)

                        tGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCg                                                >  1:88317/1‑76 (MQ=255)

                        tGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTAGTCGCATCg                                                >  1:627468/1‑76 (MQ=255)

                         ggATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCgg                                               <  1:857907/76‑1 (MQ=255)

                         ggATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCgg                                               >  1:982704/1‑76 (MQ=255)

                           aTAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGcc                                             >  2:120775/1‑76 (MQ=255)

                              gCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGGCGCATCGGcctcc                                          >  2:913790/1‑75 (MQ=255)

                               cAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGcctcgc                                         >  2:601235/1‑76 (MQ=255)

                                aaaGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGTGGTCGTCGCATCGGcctcgcc                                        <  2:899027/76‑1 (MQ=255)

                                aaaGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGcctcgcc                                        >  1:477232/1‑76 (MQ=255)

                                   ggAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGcctcgccctc                                     <  2:692602/76‑1 (MQ=255)

                                   ggAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGcctcgccctc                                     <  1:587372/76‑1 (MQ=255)

                                   ggAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGcctcgccctc                                     <  2:269967/76‑1 (MQ=255)

                                    gagaTAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGcctcgccctcg                                    <  2:239067/76‑1 (MQ=255)

                                      gaTAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCa                                  <  1:638426/76‑1 (MQ=255)

                                       aTAACGATGGGTAANACCCAGCCCTTGCCAATATGAATTACTGGCGGGGGTCGTCGCATCGGCCTCGCCCNCCCAt                                 <  2:1266010/76‑1 (MQ=255)

                                          aCGATGGGTAANACNCAGCCCTTGCCTATATGAATTACTGGCGGAGGTCGTCGCATCCTCCTCGCCCTCGCATGGc                              <  2:195628/76‑1 (MQ=255)

                                          aCGAGGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGc                              <  1:682967/76‑1 (MQ=255)

                                           cGATGGGTCAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCa                             >  1:1172854/1‑76 (MQ=255)

                                           cGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACCGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCa                             >  1:1062567/1‑76 (MQ=255)

                                             aTGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCTTCGCATCGGCCTCGCCCTCGCATGGCAtt                           <  2:540861/76‑1 (MQ=255)

                                              tGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCAttt                          >  2:824062/1‑76 (MQ=255)

                                                  tAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCCTGGCATTTCAtt                      >  1:1005419/1‑76 (MQ=255)

                                                   aaaaCCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTa                     <  2:913735/76‑1 (MQ=255)

                                                   aaaaCCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTa                     <  2:913750/76‑1 (MQ=255)

                                                   aaaaCCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTa                     >  2:1076787/1‑76 (MQ=255)

                                                   aaaaCCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTa                     <  1:530720/76‑1 (MQ=255)

                                                   aaaaCCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTa                     <  1:786142/76‑1 (MQ=255)

                                                   aaaaCCCAGCCCTTGCCAATATGAATTACTGGCGCAGGTCGTCGCCGCGGCCACGCCCTCGCATTGCGTGTCAtca                     >  2:1076781/1‑74 (MQ=255)

                                                       cccAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCa                 <  1:210815/76‑1 (MQ=255)

                                                        ccAGCCCTTGCCAATATGAATTACTGGCGGAGGTAGTCGCANCGGCCTCGCCCTCGCCCGNGANTTCATTAATCaa                >  1:1032253/1‑76 (MQ=255)

                                                             ccTTGGCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCa           >  1:81336/1‑76 (MQ=255)

                                                             ccTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCa           >  1:213816/1‑76 (MQ=255)

                                                              cTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCaa          >  2:1177890/1‑76 (MQ=255)

                                                                tGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAAcc        <  2:213968/76‑1 (MQ=255)

                                                                    aaTATGAATTACTGGCGGAGTTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTTATCAAAAGCAACCGGTg    <  1:1253188/76‑1 (MQ=255)

                                                                    aaTATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTg    >  1:1025936/1‑76 (MQ=255)

                                                                      tatGAATTACTGGCGGAGGTCGTCGCATCGGCCTAGCNCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGAt  >  1:1232336/1‑76 (MQ=255)

GTGGGATAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGAT  >  REL606/1658126‑1658271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`