Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	99.9 / NA	32	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/18);  total (14/18)

GATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGC  >  REL606/2193709‑2193849                                                                       |                                                                       gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                   <  1:896300/76‑1 (MQ=255)      aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa                                                              >  2:694102/1‑76 (MQ=255)       tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                             <  1:121960/76‑1 (MQ=255)            cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc                                                        >  2:773625/1‑76 (MQ=255)                 aGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGAATGAc                                                   >  1:866610/1‑76 (MQ=255)                 aGGCGCGGCTGGTCNGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCAGCAAATAAAGCATGAc                                                   <  1:1087977/76‑1 (MQ=255)                  ggCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg                                                  <  2:331666/76‑1 (MQ=255)                      cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                              <  1:542521/76‑1 (MQ=255)                      cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                              >  1:971022/1‑76 (MQ=255)                         cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                           >  1:990553/1‑76 (MQ=255)                            gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCACAANGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                        >  1:1234015/1‑76 (MQ=255)                            gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                        >  2:950296/1‑76 (MQ=255)                              cTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                      <  2:357929/76‑1 (MQ=255)                                ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                    >  2:364010/1‑76 (MQ=255)                                    gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                <  2:886816/76‑1 (MQ=255)                                    gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                <  1:850518/76‑1 (MQ=255)                                      cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                              <  1:666882/76‑1 (MQ=255)                                       ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                             <  2:1102016/76‑1 (MQ=255)                                       ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                             <  1:699712/76‑1 (MQ=255)                                        gAAATCAGCCCCAATAACAGTAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGGATTAAACAGGCTTTTTa                            <  1:1260372/76‑1 (MQ=255)                                         aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                           <  1:823076/76‑1 (MQ=255)                                             cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATAACGCCTGTATTAAGCCGGCTTTGTACCGAt                       >  1:28505/1‑76 (MQ=255)                                              aGCCCCAATAACAGCAAAAGGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                      >  1:1078145/1‑76 (MQ=255)                                                     aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc               >  2:237255/1‑76 (MQ=255)                                                      tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg              <  2:30234/76‑1 (MQ=255)                                                         cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc           >  2:979988/1‑76 (MQ=255)                                                          aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa          <  2:1208029/76‑1 (MQ=255)                                                           gCAAAACGCCAATCCCCACCAGATAAAGCTTGACGCCTGTATTAAACAGGCTTTTTACCGATGGTGCATCGCGCAc         <  1:64902/76‑1 (MQ=255)                                                           gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc         <  2:289443/76‑1 (MQ=255)                                                            cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc        >  2:816278/1‑76 (MQ=255)                                                             aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa       <  2:171450/76‑1 (MQ=255)                                                               aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc     >  2:36990/1‑76 (MQ=255)                                                                  gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgc  >  2:1177549/1‑76 (MQ=255)                                                                       |                                                                       GATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGC  >  REL606/2193709‑2193849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑