Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,497,881	C→T	R52H (CGT→CAT)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,497,881	0	C	T	100.0%	141.9 / NA	38	R52H (CGT→CAT)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/19);  total (19/19)

CGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGACGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAA  >  REL606/3497808‑3497956                                                                          |                                                                            cGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGa                                                                           >  2:607613/1‑76 (MQ=255)    cAGGTAGTAACCCTGACAAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCACCACGAGATGATcg                                                                        >  1:101595/1‑76 (MQ=255)    cAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATcg                                                                        <  2:228380/76‑1 (MQ=255)       gtagtaAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCgcgc                                                                     >  2:416195/1‑76 (MQ=255)           taAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAg                                                                 <  2:840150/76‑1 (MQ=255)            aaaCCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGc                                                                >  2:616593/1‑76 (MQ=255)            aaaCCTGACGAGATTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGAACAGc                                                                >  2:47381/1‑76 (MQ=255)                cTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGGTCGCGCACAGCAAAt                                                            <  2:542662/76‑1 (MQ=255)                 tGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTACGCAGCGCTCCACGNGATGAGCTCGNACACCAAATa                                                           <  2:1242018/76‑1 (MQ=255)                  gACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATaa                                                          <  2:634897/76‑1 (MQ=255)                  gACGAGTTTCTGGCCACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGTTCGCGCACAGCAAATaa                                                          <  1:98364/76‑1 (MQ=255)                        tttCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGc                                                    >  2:777522/1‑76 (MQ=255)                          tCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGt                                                  <  1:770132/76‑1 (MQ=255)                           cTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGtt                                                 >  2:807200/1‑76 (MQ=255)                             tGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCa                                               <  2:835495/76‑1 (MQ=255)                              gCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAg                                              <  1:329477/76‑1 (MQ=255)                                 aCAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCa                                           >  2:1226432/1‑76 (MQ=255)                                  cAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCAt                                          >  2:899376/1‑76 (MQ=255)                                      tGGGCACGGTATGAACGGAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGAGTTCAGCCATTCAt                                      >  1:98282/1‑76 (MQ=255)                                       gggCACGGTTTGAAGGTGATCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATg                                     <  1:55853/76‑1 (MQ=255)                                          cACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGttt                                  >  1:885259/1‑76 (MQ=255)                                          cACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGttt                                  <  1:467104/76‑1 (MQ=255)                                            cGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTAt                                <  2:473711/76‑1 (MQ=255)                                              gTTTGAATGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTg                              <  1:763683/76‑1 (MQ=255)                                              gTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTg                              >  1:614113/1‑76 (MQ=255)                                                      gTAACCAGCGCTCCTCGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTACTGGCgacgac                      <  1:104281/76‑1 (MQ=255)                                                      gTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCgacgac                      <  2:259211/76‑1 (MQ=255)                                                       tAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGAcc                     >  2:745253/1‑76 (MQ=255)                                                        aaCCCGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTTATGTTTATTGGCGACGACCg                    <  2:98282/76‑1 (MQ=255)                                                         aCCAGCGCACCACGAGATGATCGCGCACAGCATATGACGTTGCGTTGAGCTATTCATGTTTATGGGCGACGACCgg                   >  2:23561/1‑76 (MQ=255)                                                           cAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGt                 >  2:1136923/1‑76 (MQ=255)                                                            aGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCggggc                >  1:745664/1‑74 (MQ=255)                                                            aGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTc                <  2:1182755/76‑1 (MQ=255)                                                                cTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGGCCgtt            >  2:586989/1‑76 (MQ=255)                                                                 tCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTc           <  1:297978/76‑1 (MQ=255)                                                                 tCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTc           >  1:245141/1‑76 (MQ=255)                                                                     cGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCGTTTCCAAt       >  2:1123586/1‑76 (MQ=255)                                                                      gagaTGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATc      <  2:413738/76‑1 (MQ=255)                                                                       agaTGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCg     >  2:265460/1‑76 (MQ=255)                                                                          cGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTaa  <  2:337414/76‑1 (MQ=255)                                                                          |                                                                            CGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGACGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAA  >  REL606/3497808‑3497956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑