Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	490,300	IS150 (+) +3 bp	coding (146‑148/855 nt)	ybbN ←	predicted thioredoxin domain‑containing protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	490300 =	0 (0.000)	46 (1.070)	35/150	0.3	100%	coding (148/855 nt)	ybbN	predicted thioredoxin domain‑containing protein
	?	REL606	= 666130	NA (NA)					noncoding (1443/1443 nt)	IS150	repeat region
*	?	REL606	= 490302	0 (0.000)	60 (1.390)	45/150	0.0	100%	coding (146/855 nt)	ybbN	predicted thioredoxin domain‑containing protein
	?	REL606	588495 =	NA (NA)					noncoding (1/1977 nt)	IS150	repeat region

CGGTGCTGTTCTATTTTTGGTCTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/490374‑490300 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGA  <  REL606/666130‑666056                                                                                                                                                        CGGTGCTGTTCTATTTTTGGTCTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGT                                                                            >  1:394116/1‑76      CTGTTCTATTTTTGGTCTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACT                                                                       <  1:1117449/76‑1       TGTTCTATTTTTGGTCTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTG                                                                      <  2:559767/76‑1          TCTATTTTTGGTCTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACC                                                                   >  1:1338324/1‑76          TCTATTTTTGGTCTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCATCGCGGTGTACTGACC                                                                   >  1:1338332/1‑76           CTATTTTTGGTCTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCC                                                                  >  2:822359/1‑76            TATTTTTGGTCTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCC                                                                 >  1:816127/1‑76             ATTTTTGGTCTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCA                                                                <  1:806097/76‑1              TTTTTGGTCTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAA                                                               <  1:401338/76‑1                TTTGGTCTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAA                                                             <  1:1282359/76‑1                TTTGGTCTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAA                                                             <  2:659354/76‑1                 TTGGTCTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAA                                                            >  2:112796/1‑76                   GGTCTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGT                                                          >  2:346792/1‑76                      CTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGG                                                       <  1:1055563/76‑1                            GTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTT                                                 <  1:754838/76‑1                             TAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTA                                                <  2:499785/76‑1                             TAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTA                                                >  2:653666/1‑76                             TAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTA                                                <  2:756986/76‑1                               GCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAA                                              <  2:903943/76‑1                                CCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAAC                                             <  2:973581/76‑1                                 CAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACA                                            >  2:704158/1‑76                                     ACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAG                                        >  2:1014360/1‑76                                     ACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAG                                        <  2:375148/76‑1                                      CTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGG                                       <  1:589053/76‑1                                       TGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGC                                      >  2:1223373/1‑76                                       TGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGC                                      >  2:1223391/1‑76                                       TGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGC                                      >  1:18054/1‑76                                          TTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATA                                   <  2:468220/76‑1                                           TGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTTGACAGTTAAACCAGAGGCATAa                                  >  2:815694/1‑75                                           TGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATAT                                  >  2:815646/1‑76                                           TGCAGTTAACCCCGATCCTGGAAAGCCTCGCGGAGTACTGAACCCAAAAAGTTTTACAGTTAAACCACAGGCATAc                                  >  2:815714/1‑75                                             CAGTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAG                                >  2:1032375/1‑76                                               GTTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGT                              <  1:1107533/76‑1                                                TTAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                             >  2:83138/1‑76                                                 TAACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCT                            <  2:164811/76‑1                                                  AACCCCGATCCTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTG                           >  1:388298/1‑76                                                            CTGGAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATAT                 >  2:1246587/1‑76                                                               GAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCA              <  2:1171277/76‑1                                                               GAAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCA              <  2:1171269/76‑1                                                                AAAGCCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAA             >  1:562143/1‑76                                                                    CCTCGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGG         <  1:159082/76‑1                                                                       CGCGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGT      >  1:209428/1‑76                                                                         CGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCA    >  1:51894/1‑76                                                                         CGGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCA    >  1:333065/1‑76                                                                           GTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGA  >  2:55195/1‑76                                                                           GTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGA  <  1:836839/76‑1                                                                                                                                                        CGGTGCTGTTCTATTTTTGGTCTGAACGTAGCCAGCACTGTTTGCAGTTAACCCCGATCCTGGAAAGCCTCGCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/490374‑490300 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGA  <  REL606/666130‑666056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.