Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	474,632	T→C	E225G (GAG→GGG)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	474,632	0	T	C	100.0%	121.4 / NA	31	E225G (GAG→GGG)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/17);  total (14/17)

AGACATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAA  >  REL606/474561‑474701                                                                        |                                                                      agaCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCcgcg                                                                   >  1:90457/1‑76 (MQ=255)  gaCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCga                                                                  >  1:863369/1‑76 (MQ=255)  gaCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCga                                                                  <  2:455139/76‑1 (MQ=255)   aCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCgag                                                                 <  2:927031/76‑1 (MQ=255)   aCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCgag                                                                 >  2:1073567/1‑76 (MQ=255)     aTCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCGAGaa                                                               >  2:236317/1‑76 (MQ=255)           cAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCGAGAACCGGtt                                                         >  2:791678/1‑76 (MQ=255)           cAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCGAGAACCGGtt                                                         <  1:907951/76‑1 (MQ=255)            aGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCGAGAACCGGTTg                                                        <  2:926257/76‑1 (MQ=255)            aGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCACCAGCACCGACAGGGTAAACAGCCCGCGAGAACCGGTTg                                                        >  2:690101/1‑76 (MQ=255)                     gCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGc                                               <  2:31446/76‑1 (MQ=255)                          aaaGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGcc                                          >  1:812233/1‑76 (MQ=255)                                aaCCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATc                                    <  2:1018072/76‑1 (MQ=255)                                   cccTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCAc                                 <  1:1094185/76‑1 (MQ=255)                                   cccTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCAc                                 <  2:1223630/76‑1 (MQ=255)                                      tAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGc                              >  2:236309/1‑76 (MQ=255)                                      tAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGc                              >  2:534031/1‑76 (MQ=255)                                      tAACGCCAGCGCCAGCACCGACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGc                              <  2:740142/76‑1 (MQ=255)                                             agcgccagcACCGACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGAc                       >  1:1204820/1‑76 (MQ=255)                                               cgccagcACCGACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACgg                     >  2:999656/1‑76 (MQ=255)                                                 ccagcACCGACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCg                   <  2:501346/76‑1 (MQ=255)                                                  cagcACCGACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGa                  >  1:36343/1‑76 (MQ=255)                                                     cACCGACGGGGTAAACAGCCCGCGAGAACGGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCt               <  2:1151710/76‑1 (MQ=255)                                                      cccGACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTa              <  2:151556/75‑1 (MQ=255)                                                       ccGACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTAc             >  1:231392/1‑76 (MQ=255)                                                        cGACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTAcc            <  1:267838/76‑1 (MQ=255)                                                         gACAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCa           <  2:505345/76‑1 (MQ=255)                                                           cAGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGc         <  1:699356/76‑1 (MQ=255)                                                            aGGGTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGca        <  1:1353030/76‑1 (MQ=255)                                                              ggTAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGcatc      <  1:1265140/76‑1 (MQ=255)                                                                tAAACAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGcatcat    >  1:939665/1‑76 (MQ=255)                                                                  aaCAGCCCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATaa  <  2:1197664/76‑1 (MQ=255)                                                                        |                                                                      AGACATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATAA  >  REL606/474561‑474701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑