Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,305,101	G→T	A380D (GCC→GAC)	cls ←	cardiolipin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,305,101	0	G	T	100.0%	105.1 / NA	32	A380D (GCC→GAC)	cls	cardiolipin synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/19);  total (13/19)

ATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGTG  >  REL606/1305028‑1305167                                                                          |                                                                   aTGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTcc                                                                  >  1:355751/1‑76 (MQ=255)  tGCAGTACCCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTccc                                                                 <  1:46899/76‑1 (MQ=255)    cAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAg                                                               <  1:697974/76‑1 (MQ=255)    cAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAg                                                               >  2:294313/1‑76 (MQ=255)      gTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGcc                                                             >  1:681039/1‑76 (MQ=255)      gTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGcc                                                             <  2:419625/76‑1 (MQ=255)       tAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCg                                                            >  1:340222/1‑76 (MQ=255)       naaCCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCg                                                            >  1:430408/2‑76 (MQ=255)        aaCCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCGa                                                           >  2:1137149/1‑76 (MQ=255)          cccGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCGAcc                                                         <  1:560535/76‑1 (MQ=255)             gCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGc                                                      <  2:111823/76‑1 (MQ=255)                ttCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATc                                                   <  1:766304/76‑1 (MQ=255)                ttCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATc                                                   <  1:859604/76‑1 (MQ=255)                ttCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATc                                                   <  1:103001/76‑1 (MQ=255)                   aaaCTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATCGAg                                                >  2:1257442/1‑76 (MQ=255)                          tAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATCGAGTCAtttt                                         >  2:1164688/1‑76 (MQ=255)                                 tAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCgg                                  >  2:544271/1‑76 (MQ=255)                                 tAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCgg                                  >  1:1352990/1‑76 (MQ=255)                                 tAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCgg                                  >  1:1145200/1‑76 (MQ=255)                                 tAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCgg                                  >  2:1092173/1‑76 (MQ=255)                                   aCCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGaa                                <  2:629453/76‑1 (MQ=255)                                    ccccAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAg                               <  2:789209/76‑1 (MQ=255)                                         gcagccagcagTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGataa                          <  1:710799/76‑1 (MQ=255)                                         gcagccagcagTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGataa                          <  1:171777/76‑1 (MQ=255)                                                 cagTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACa                  <  1:61209/76‑1 (MQ=255)                                                   gTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATc                <  1:815680/76‑1 (MQ=255)                                                   gTTCCGTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATc                <  2:1229052/76‑1 (MQ=255)                                                        gTAAAGAATGCGCGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCAccc           <  2:1193415/76‑1 (MQ=255)                                                            aGAATGCGCGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCcgcg       >  1:1091010/1‑76 (MQ=255)                                                              aaTGCGCGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCt     <  1:379141/76‑1 (MQ=255)                                                                tGCGCGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCtgt   <  2:942479/76‑1 (MQ=255)                                                                 gcgcGACTGTCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCtgtg  <  2:662846/76‑1 (MQ=255)                                                                          |                                                                   ATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGTG  >  REL606/1305028‑1305167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑