Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,414,552	T→G	I176M (ATT→ATG)	crp →	DNA‑binding transcriptional dual regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,414,552	0	T	G	100.0%	146.4 / NA	36	I176M (ATT→ATG)	crp	DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/19);  total (17/19)

CAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACA  >  REL606/3414479‑3414623                                                                          |                                                                        cAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGt                                                                       >  1:755627/1‑76 (MQ=255)         ccAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGtt                                                               <  2:497292/76‑1 (MQ=255)               gCTGTGACTCACCCGGCCGGTATGCAAATCAAAATGACCCGTCAGGAAATTGGTCAGAGGGTCGGCTGTTCTCGTg                                                         <  1:1140660/76‑1 (MQ=255)               gCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTg                                                         <  1:1044685/76‑1 (MQ=255)               gCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTg                                                         <  2:184907/76‑1 (MQ=255)               gCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTg                                                         <  1:1140639/76‑1 (MQ=255)                    gACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAAcc                                                    >  1:661920/1‑76 (MQ=255)                     aCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCg                                                   >  1:82675/1‑76 (MQ=255)                     aCTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCg                                                   >  1:72261/1‑76 (MQ=255)                        cACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTgg                                                <  2:482430/76‑1 (MQ=255)                            cGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACg                                            <  1:437966/76‑1 (MQ=255)                            cGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACg                                            >  2:928048/1‑76 (MQ=255)                                  gTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCt                                      >  1:748292/1‑76 (MQ=255)                                  gTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCt                                      >  1:981933/1‑76 (MQ=255)                                    aTGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTTTGa                                    <  2:516843/76‑1 (MQ=255)                                     tGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGaa                                   >  1:107703/1‑76 (MQ=255)                                      gCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAg                                  >  1:1009966/1‑76 (MQ=255)                                        aaaTCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGAt                                <  2:768333/76‑1 (MQ=255)                                         aaTCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATg                               >  1:1305004/1‑76 (MQ=255)                                           tCAAAATTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCt                             >  1:366336/1‑76 (MQ=255)                                              aaaTTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGa                          <  2:546124/76‑1 (MQ=255)                                               aaTTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGaa                         <  2:1107468/76‑1 (MQ=255)                                                aTTACCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAg                        >  2:120398/1‑76 (MQ=255)                                                   aCCCGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc                     >  1:625123/1‑76 (MQ=255)                                                    cccGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCa                    >  2:590353/1‑76 (MQ=255)                                                    cccGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCa                    <  1:905616/76‑1 (MQ=255)                                                    cccGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCa                    <  1:905629/76‑1 (MQ=255)                                                     ccGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAg                   <  1:514359/76‑1 (MQ=255)                                                     ccGTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGGGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAg                   <  1:514351/76‑1 (MQ=255)                                                       gTCAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGaa                 >  1:536192/1‑76 (MQ=255)                                                         cAGGAAATTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAAcc               >  2:575862/1‑76 (MQ=255)                                                             aaaTTGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAt           <  1:1057204/76‑1 (MQ=255)                                                                ttGGTCAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAtctc        >  1:1161642/1‑76 (MQ=255)                                                                     cAGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGcac   <  2:747835/76‑1 (MQ=255)                                                                      aGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGcaca  <  2:261575/76‑1 (MQ=255)                                                                      aGATGGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGcaca  <  1:1344218/76‑1 (MQ=255)                                                                          |                                                                        CAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACA  >  REL606/3414479‑3414623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑