Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,512,474	C→T	intergenic (‑216/‑117)	yhhX ← / → yhhY	predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/predicted acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,512,474	0	C	T	100.0%	176.1 / NA	48	intergenic (‑216/‑117)	yhhX/yhhY	predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/predicted acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/29);  total (19/29)

TAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAG  >  REL606/3512401‑3512546                                                                          |                                                                         tAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATaa                                                                        <  1:1257477/76‑1 (MQ=255)   aGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGataata                                                                      <  1:1286189/76‑1 (MQ=255)   aGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATaatn                                                                      <  1:253353/76‑2 (MQ=255)    gTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGAtaataa                                                                     <  2:1227894/76‑1 (MQ=255)    gTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGAtaataa                                                                     <  2:501285/76‑1 (MQ=255)    gTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGAtaataa                                                                     >  1:273896/1‑76 (MQ=255)     tAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATaataat                                                                    <  2:126847/76‑1 (MQ=255)     tAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATaataat                                                                    <  1:1290122/76‑1 (MQ=255)      aaTACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGataataata                                                                   >  2:1305160/1‑76 (MQ=255)       aTACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGAtaataataa                                                                  <  1:1230808/76‑1 (MQ=255)       aTACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGAtaataataa                                                                  <  2:784801/76‑1 (MQ=255)        tACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATaataataat                                                                 <  1:105361/76‑1 (MQ=255)         aCTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGCCTTCCTGATCGCGAGATAATAATAAtc                                                                >  1:780105/1‑76 (MQ=255)           tGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAAtcat                                                              <  2:1301047/76‑1 (MQ=255)               aGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAAtcattctc                                                          >  2:236774/1‑76 (MQ=255)               aGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAAtcattctc                                                          >  1:1087916/1‑76 (MQ=255)               aGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAAtcattctc                                                          >  1:1087910/1‑76 (MQ=255)                gCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAAtcattctca                                                         <  2:1035174/76‑1 (MQ=255)                 cAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAAtcattctcat                                                        <  2:156075/76‑1 (MQ=255)                   aTGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAAtcattctcattc                                                      <  2:970145/76‑1 (MQ=255)                    tgtgAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCg                                                     <  2:631247/76‑1 (MQ=255)                      tgAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCa                                                   <  2:120358/76‑1 (MQ=255)                         gCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACtt                                                >  1:447589/1‑76 (MQ=255)                          cAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTg                                               <  2:806834/76‑1 (MQ=255)                          cAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTg                                               >  2:383234/1‑76 (MQ=255)                           aaTGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGt                                              <  2:837860/76‑1 (MQ=255)                           aaTGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGt                                              <  2:837845/76‑1 (MQ=255)                            aTGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTc                                             <  2:284842/76‑1 (MQ=255)                              gTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCa                                           <  2:846649/76‑1 (MQ=255)                              gTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCa                                           >  2:650838/1‑76 (MQ=255)                              gTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCa                                           <  1:262111/76‑1 (MQ=255)                                cGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAac                                         >  2:239937/1‑76 (MQ=255)                                 gTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAaca                                        <  1:641401/76‑1 (MQ=255)                                    cTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACtt                                     <  1:515221/76‑1 (MQ=255)                                       tCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACttttt                                  >  2:77062/1‑76 (MQ=255)                                       tCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACttttt                                  >  1:92022/1‑76 (MQ=255)                                        cAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTg                                 >  1:824532/1‑76 (MQ=255)                                          ggTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCa                               <  1:90512/76‑1 (MQ=255)                                          ggTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCa                               <  1:601581/76‑1 (MQ=255)                                           gTTCTCCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCaa                              >  2:888281/1‑76 (MQ=255)                                             tctcCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCaaaa                            <  1:292176/76‑1 (MQ=255)                                             tctcCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCaaaa                            <  2:903604/76‑1 (MQ=255)                                              ctcCGCGAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCaaaaa                           >  1:1288666/1‑76 (MQ=255)                                                 cgcgAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAAt                        >  1:72328/1‑76 (MQ=255)                                                  gcgAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATg                       <  1:123446/76‑1 (MQ=255)                                                    gAGGGTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCa                     >  1:177176/1‑76 (MQ=255)                                                       ggTCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCAttt                  <  1:283557/76‑1 (MQ=255)                                                         tCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGa                <  2:1051497/76‑1 (MQ=255)                                                         tCTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGa                >  1:850895/1‑76 (MQ=255)                                                          cTTCCTGATCGCGCGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGAc               >  1:253378/1‑76 (MQ=255)                                                          cTTCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGAc               >  2:1212015/1‑76 (MQ=255)                                                            tCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTc             <  2:1095857/76‑1 (MQ=255)                                                            tCCTGATCGCGAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTc             >  2:950811/1‑76 (MQ=255)                                                                   cgcgAGATAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTg      <  1:898240/76‑1 (MQ=255)                                                                      gagaTAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGaaa   <  2:45884/76‑1 (MQ=255)                                                                       agaTAATAATAATAATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAg  >  2:847608/1‑76 (MQ=255)                                                                          |                                                                         TAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAG  >  REL606/3512401‑3512546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑