| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 474,189 | +GC | coding (1117/1677 nt) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 474,186 | 1 | . | C | 78.6% | 83.4 / 20.4 | 28 | coding (1120/1677 nt) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base . (3/3); new base C (12/10); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01 | |||||||||||
| * | REL606 | 474,186 | 2 | . | G | 78.6% | 81.4 / 20.7 | 28 | coding (1120/1677 nt) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base . (3/3); new base G (12/10); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.85e-01 | |||||||||||
TCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAT‑‑CGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCG > REL606/474113‑474251 || tCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAT‑‑Cg > 2:267018/1‑76 (MQ=255)tCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAT‑‑Cg > 1:138930/1‑76 (MQ=255)tCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAT‑‑Cg < 1:647752/76‑1 (MQ=255) tttCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAT‑‑CGcgcccct < 2:648279/76‑4 (MQ=255) tttCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAT‑‑CGcgcccct < 1:318003/76‑4 (MQ=255) tttCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAT‑‑CGcgcccct > 1:192091/1‑73 (MQ=255) ggTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCa < 1:35444/76‑1 (MQ=255) ggTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCa > 1:847174/1‑76 (MQ=255) ggTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCa > 1:847181/1‑76 (MQ=255) ggTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCa < 2:1049513/76‑1 (MQ=255) gTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAg > 2:1056073/1‑76 (MQ=255) tCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATGATCGACAGGATCGCGCCCCTGCCAGt > 2:752351/1‑76 (MQ=255) cTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTa < 1:984412/76‑1 (MQ=255) ttCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTAc > 2:902940/1‑76 (MQ=255) ccAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTACCAgg > 2:482180/1‑76 (MQ=255) ccAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTACCAgg > 1:842472/1‑76 (MQ=255) tttCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTACCAGGTTTTGTc < 1:1150361/76‑1 (MQ=255) ccAGTAGTGCGCACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTACCAGGTTTTGTCCGGcc > 1:718094/1‑76 (MQ=255) cAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCt < 1:1137648/76‑1 (MQ=255) agtagtGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTg < 2:565603/76‑1 (MQ=255) gtagtGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGc > 2:632389/1‑76 (MQ=255) gtGCGAACATTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGc < 2:435308/76‑1 (MQ=255) gtGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGc > 2:692639/1‑76 (MQ=255) gtGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGc < 1:75214/76‑1 (MQ=255) accAGTCCCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTaa < 2:393230/74‑1 (MQ=255) cAGTACCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAAt < 1:98391/76‑1 (MQ=255) aCCGGGTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCaa < 2:1092932/76‑1 (MQ=255) gTTGAGCATAATCGACAGGATCGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCa > 2:1005058/1‑76 (MQ=255) ttGAGCATAATCGACAGGATCGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCAt > 1:946652/1‑76 (MQ=255) tAATCGACAGGATCGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGt > 2:208305/1‑76 (MQ=255) cGACAGGATCGCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCg > 1:804868/1‑76 (MQ=255) cGACAGGATCGCGCCCCTGCCAGTACCAGGTTTCGTCCGGGCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCg < 2:989882/76‑1 (MQ=255) || TCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGAT‑‑CGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCG > REL606/474113‑474251 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |