| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 154,497 | 0 | T | G | 36.4% | 57.7 / 23.5 | 44 | A191A (GCA→GCC) | yadL | predicted fimbrial‑like adhesin protein |
| Reads supporting (aligned to +/- strand): ref base T (1/27); new base G (16/0); total (17/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.08e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGATAA > REL606/154425‑154572 | gTTGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCa > 1:1133229/1‑76 (MQ=255) tGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGa < 1:132565/76‑1 (MQ=255) tGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGa > 1:506575/1‑76 (MQ=255) gAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGaa < 2:826169/76‑1 (MQ=255) gAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTATAAATGTGGCAGGTGCAGaa < 1:253977/76‑1 (MQ=255) aGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGaaa < 1:725647/76‑1 (MQ=255) gTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGaaaa < 2:56516/76‑1 (MQ=255) gTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGaaaa < 2:131567/76‑1 (MQ=255) tGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAAt > 1:946460/1‑76 (MQ=255) tGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAAt > 1:780716/1‑76 (MQ=255) gTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACa < 1:905454/76‑1 (MQ=255) ccTGGATCTGTCAGTCTGTTAGTTAGAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTg < 1:246525/76‑1 (MQ=255) ggATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCtt < 1:270340/76‑1 (MQ=255) ggATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAAATTACCCGCAGTGACATTGCtt > 1:1069584/1‑76 (MQ=255) gATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTa < 2:309006/76‑1 (MQ=255) gATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTa < 1:494636/76‑1 (MQ=255) gATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTa < 1:233040/76‑1 (MQ=255) gATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTa < 2:603971/76‑1 (MQ=255) tGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGt < 2:930505/76‑1 (MQ=255) gTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTTCgggt > 1:833172/1‑74 (MQ=255) aGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTa < 2:327002/76‑1 (MQ=255) cTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTACGGtttttttt > 1:782858/1‑76 (MQ=255) gttagttaTAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTAttttt < 1:913428/76‑1 (MQ=255) gttagttaTAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTTCGGGTtttttttt > 1:329577/1‑76 (MQ=255) agttaTAGGTTACTGTAAATGTGGCAGGGGCCGAAAAATTACCCGCAGTGACATTGCTTACGGttttttttttttt > 1:33895/1‑75 (MQ=255) agttaTAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTTCGGGTTTAtttttttt > 1:708625/1‑75 (MQ=255) gttaTAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTcg < 2:812324/76‑1 (MQ=255) gttaTAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTTCGGTTTTATTTTTTTcg > 2:434695/1‑76 (MQ=255) ttaTAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTcga < 1:206282/76‑1 (MQ=255) ttACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAg < 1:926777/76‑1 (MQ=255) ttACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTTCGGGTTTTTTTTTTTTGTCGAACAg > 2:291781/1‑76 (MQ=255) ttACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTACGGGTTTTTTTTTTTTGACGACCAg > 2:858730/1‑76 (MQ=255) tACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTTTTTTTTTTGACGACCAGt > 1:720719/1‑76 (MQ=255) cTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCg < 2:1147106/76‑1 (MQ=255) cTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCg < 2:1139404/76‑1 (MQ=255) cTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCg < 2:643958/76‑1 (MQ=255) cTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCg < 2:826230/76‑1 (MQ=255) tGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTACGGGTTTATTTTTTTTTACGACCCGTCGa > 2:56519/1‑76 (MQ=255) tAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTTGACGACCCGTCGAGc > 2:859200/1‑76 (MQ=255) aaTGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTACGGGTTTTTTTTTTTTGACGACCAGTCGAGCAc > 2:782603/1‑76 (MQ=255) aaTGTGGCAGGGGCAGAAAATTTACCCGCAGGGACATTGCTTACGGTTTTATTTTTTTCGCCGACCCATCGAGCAc > 1:90500/1‑76 (MQ=255) tgtgGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTc < 1:1146581/76‑1 (MQ=255) gtgGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCa > 2:364492/1‑76 (MQ=255) tgGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCAt < 2:19103/76‑1 (MQ=255) cAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCgg < 1:94341/76‑1 (MQ=255) aGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGa < 1:647464/76‑1 (MQ=255) tGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGataa < 2:675998/76‑1 (MQ=255) tGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGataa < 2:938755/76‑1 (MQ=255) | GTTGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGATAA > REL606/154425‑154572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |