| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,484,105 | 0 | A | G | 32.0% | 32.3 / 11.6 | 25 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (4/13); new base G (8/0); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAA > REL606/1484031‑1484179 | ttACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAt < 1:1114683/76‑1 (MQ=255)ttACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAt < 1:970918/76‑1 (MQ=255) gctgctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGtttttt > 1:602562/1‑74 (MQ=255) gctgctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtat < 2:876368/76‑1 (MQ=255) ctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACtt < 1:644683/76‑1 (MQ=255) tGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTTCtttt > 2:775766/1‑76 (MQ=255) ggCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTg < 1:219862/76‑1 (MQ=255) cccACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTAAttttttc > 1:109719/1‑73 (MQ=255) tAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTTCTTTTTTCGAttt > 2:59511/1‑76 (MQ=255) aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTACGGtttt > 1:736095/1‑75 (MQ=255) gtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGATACTGTTATGGCAGGGGTTTTTTTCTTTTTTCGATTTTAcc > 1:1036646/1‑76 (MQ=255) ggTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCAt < 2:58979/76‑1 (MQ=255) gTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTCCCATg > 2:247738/1‑76 (MQ=255) aTTGTCTATGTCGGCTCGCTGCTGACACTGTTATGGCTGGGATTTTATACTTTTGACGATTTCACCATGTCGGTaa < 1:225796/76‑1 (MQ=255) cTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTTCGATTTTACCCTGTCGGGAAAGccc > 1:829138/1‑75 (MQ=255) tCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACt < 2:821219/76‑1 (MQ=255) ggCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTCGGTAACCCCGGAACTGa > 2:991135/1‑76 (MQ=255) cTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACg < 1:824898/76‑1 (MQ=255) tgctgACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCCGGc > 2:587820/1‑76 (MQ=255) gctgACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTCGGTCACGCCGGAACTGACGCTGGCg > 2:718424/1‑76 (MQ=255) gACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAat < 2:691991/76‑1 (MQ=255) acacTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAata < 1:89210/76‑1 (MQ=255) cacTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAatat < 2:479131/76‑1 (MQ=255) cTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCCTGTCAGTAACGCCGGAACTGACGCTGGCGAATATcc > 2:78776/1‑76 (MQ=255) ttATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGGAACGCCGGAACTGACGCCGGCGAATATCCGTg > 2:352532/1‑76 (MQ=255) cAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGt > 1:576630/1‑76 (MQ=255) cAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCACTGCGCTGt > 1:295005/1‑76 (MQ=255) gATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTaa < 2:89750/76‑1 (MQ=255) | TTACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAA > REL606/1484031‑1484179 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |