| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,163,734 | T→C | L960P (CTG→CCG) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,163,734 | 0 | T | C | 100.0% | 68.2 / NA | 18 | L960P (CTG→CCG) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/6); total (12/6) | |||||||||||
CGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGC > REL606/4163663‑4163804 | cgATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCCGTCt < 1:370470/76‑1 (MQ=255) tGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCCGTCTgaa < 2:472744/76‑1 (MQ=255) ggCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCCGTCTggag < 1:296563/76‑1 (MQ=255) gCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCCGTCTgaaga < 1:3347/76‑1 (MQ=255) aGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCCGTCTGAAGAACTg > 2:773522/1‑76 (MQ=255) aaaGACAAACGTGCGCTGGAAATCAAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCCGTCTGAAGAACTGCAGa > 1:709580/1‑76 (MQ=255) gcgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCCGTCTGAAGAACTGCAGATCCTCGAAGCgg < 2:627346/76‑1 (MQ=255) ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCCGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTg > 2:26248/1‑76 (MQ=255) ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCCGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTg > 2:1083417/1‑76 (MQ=255) cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCCGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGc > 2:944437/1‑76 (MQ=255) gCTCAAACAGGCGAAGAAAGACCCGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgct > 1:231321/1‑76 (MQ=255) cTCAAACAGGCGAAGAAAGACCCGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctg > 1:828616/1‑76 (MQ=255) gggCGAAGAAAGACCCGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt < 2:85691/75‑1 (MQ=255) aGGCGAAGAAAGCCCCGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt < 2:85684/76‑1 (MQ=255) cGAAGAAAGACCCGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGc > 1:427013/1‑76 (MQ=255) gaagaaAGACCCGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGcc > 1:1153045/1‑76 (MQ=255) agaaagaCCCGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCgg > 2:313697/1‑76 (MQ=255) aagaCCCGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg > 2:283392/1‑76 (MQ=255) agaCCCGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGc > 1:906773/1‑76 (MQ=255) | CGATGGCGTAGAAAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGC > REL606/4163663‑4163804 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |