| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 702,967 | A→G | intergenic (+32/+184) | ECB_00650 → / ← kdpE | conserved hypothetical protein/DNA‑binding response regulator in two‑component regulatory system with KdpD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 702,967 | 0 | A | G | 98.2% | 219.3 / NA | 54 | intergenic (+32/+184) | ECB_00650/kdpE | conserved hypothetical protein/DNA‑binding response regulator in two‑component regulatory system with KdpD |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (27/26); minor base T (0/1); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGTTTTTTAC > REL606/702896‑703039 | tAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATa < 2:317420/76‑1 (MQ=255) cGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGtt < 2:492181/76‑1 (MQ=255) gTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACt < 1:617379/76‑1 (MQ=255) cATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTg < 2:808724/76‑1 (MQ=255) atttaatttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGa < 2:452994/76‑1 (MQ=255) atttaatttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGa < 2:1115911/76‑1 (MQ=255) ttaatttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATc > 1:1059157/1‑76 (MQ=255) taatttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCa > 1:373523/1‑76 (MQ=255) taatttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCa < 1:44332/76‑1 (MQ=255) taatttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCa > 1:1069076/1‑76 (MQ=255) aatttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCaa < 2:1121355/76‑1 (MQ=255) atttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAAt < 2:1003182/76‑1 (MQ=255) atttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAAt > 2:801267/1‑76 (MQ=255) tttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATa > 2:1007999/1‑76 (MQ=255) ttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGTAATACGGTTGAACTGTGAAGGTGATCAATaa < 1:177981/76‑1 (MQ=255) ttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATaa < 1:783829/76‑1 (MQ=255) taCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATaaa < 2:53046/76‑1 (MQ=255) ccTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATaaaaa < 1:832251/76‑1 (MQ=255) ttCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATg > 1:1121750/1‑76 (MQ=255) ttCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATg > 2:57661/1‑76 (MQ=255) tCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGa > 1:361916/1‑76 (MQ=255) cTGATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATc > 1:697550/1‑76 (MQ=255) gATGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCaa > 1:115643/1‑76 (MQ=255) aTGTGAATGAAGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAAt > 1:915791/1‑76 (MQ=255) tgaatgaaGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCtt < 2:166765/76‑1 (MQ=255) gaatgaaGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTa > 1:657783/1‑76 (MQ=255) gaatgaaGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTa > 1:611514/1‑76 (MQ=255) tgaaGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTCatt > 2:956854/1‑76 (MQ=255) tgaaGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAatt > 2:956856/1‑76 (MQ=255) gaaGGTGAAGATATTCTATCCGGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAattt < 2:690609/76‑1 (MQ=255) gaaGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAattt > 1:775031/1‑76 (MQ=255) gaaGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAattt > 1:1157366/1‑76 (MQ=255) gaaGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAattt < 2:690601/76‑1 (MQ=255) aGGTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAatttat < 2:543693/76‑1 (MQ=255) gTGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAatttattt < 1:447140/76‑1 (MQ=255) tGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATTTTAAtttattta < 1:241427/76‑1 (MQ=255) tGAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAAtttattta < 2:35462/76‑1 (MQ=255) gAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTaa < 1:861022/76‑1 (MQ=255) gAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTaa > 2:485634/1‑76 (MQ=255) gAAGATATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTaa > 2:485629/1‑76 (MQ=255) gAAGAGATTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTaa < 1:861014/76‑1 (MQ=255) tatTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAatgatg < 1:795094/76‑1 (MQ=255) tatTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAatgatg < 1:500549/76‑1 (MQ=255) atTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAAtgatga > 1:690944/1‑76 (MQ=255) atTCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAAtgatga > 1:1119596/1‑76 (MQ=255) ttCTATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAg > 1:709417/1‑76 (MQ=255) tATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGttt > 1:497680/1‑76 (MQ=255) tATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGttt > 1:1002114/1‑76 (MQ=255) tATCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTAAATTTATTTAATGATGAGttt > 1:497674/1‑76 (MQ=255) aTCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGtttt < 1:1026286/76‑1 (MQ=255) tCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGttttt < 2:283959/76‑1 (MQ=255) tCCAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGttttt < 2:283953/76‑1 (MQ=255) ccAGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATATTAATTTATTTAATGATGAGtttttt > 1:927998/1‑76 (MQ=255) aGGGAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGTTTTTTAc > 2:996197/1‑76 (MQ=255) | TAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGTTTTTTAC > REL606/702896‑703039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |