Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,264,346	C→T	S161S (AGC→AGT)	hemA →	glutamyl‑tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,264,346	0	C	T	100.0%	58.2 / NA	17	S161S (AGC→AGT)	hemA	glutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/10);  total (7/10)

GGAACGCATGTTCCAGAAATCTTTCTCTGTAGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGATCTTTGAATCGCTCTCTACGGTC  >  REL606/1264277‑1264412                                                                      |                                                                   ggAACGCATGTTCCAGAAATCTTTCTCTGTAGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGTGCtgtg                                                              >  1:341845/1‑76 (MQ=255)     cGCATGTTCCAGAAATCTTTCTCTGTAGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGTGCTGtgtctg                                                          >  2:1009945/1‑76 (MQ=255)        aTGTTCCAGAAATCTTTCTCTGTAGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGTGCTGTGTCTGTCg                                                       >  1:812600/1‑76 (MQ=255)                       ttCTCTGTAGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGTGCTGTGTCTGTCGCTTTTGCGGCTTGTa                                        <  2:289051/76‑1 (MQ=255)                           ctGTAGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGTGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCt                                    <  1:745313/76‑1 (MQ=255)                            tGTAGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGTGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTg                                   >  1:1048138/1‑76 (MQ=255)                             gTAGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGTGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTgg                                  <  2:1114254/76‑1 (MQ=255)                              tAGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGTGCTGTGTCTGTCGCTTTTGCGGCTTGTTCGCCggg                                 >  2:848278/1‑75 (MQ=255)                              tAGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGTGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCCggg                                 >  2:960579/1‑75 (MQ=255)                                       gcgTTCGCACTGAAACAGATATCGGTGCCAGTGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGAt                        <  2:864142/76‑1 (MQ=255)                                        cgTTCGCACTGAAACAGATATCGGTGCCAGTGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGATc                       <  2:645349/76‑1 (MQ=255)                                         gTTCGCACTGAAACAGATATCGGTGCCAGTGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGATCt                      <  1:31176/76‑1 (MQ=255)                                            cGCACTGAAACAGATATCGGTGCCAGTGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGATCTTTg                   <  2:341624/76‑1 (MQ=255)                                            cGCACTGAAACAGATATCGGTGCCAGTGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGATCTTTg                   <  2:801680/76‑1 (MQ=255)                                                          tatCGGTGCCAGTGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGATCTTTGAATCGCTCTCTACg     <  1:843459/76‑1 (MQ=255)                                                            tCGGTGCCAGTGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGATCTTTTAATTGCTCTCTTCGGt   >  1:957522/1‑76 (MQ=255)                                                             cGGTGCCAGTGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGATCTTTGAATCGCTCTCTACGGTc  <  1:190930/76‑1 (MQ=255)                                                                      |                                                                   GGAACGCATGTTCCAGAAATCTTTCTCTGTAGCGAAACGCGTTCGCACTGAAACAGATATCGGTGCCAGCGCTGTGTCTGTCGCTTTTGCGGCTTGTACGCTGGCGCGGCAGATCTTTGAATCGCTCTCTACGGTC  >  REL606/1264277‑1264412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑