Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,928,343	G→A	A43T (GCA→ACA)	rho →	transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,928,343	0	G	A	100.0%	88.6 / NA	24	A43T (GCA→ACA)	rho	transcription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/11);  total (13/11)

CGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTT  >  REL606/3928270‑3928415                                                                          |                                                                         cGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGcacaca                                                                        <  2:657850/76‑1 (MQ=255)    aaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAag                                                                     >  1:93740/1‑76 (MQ=255)              cTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAg                                                           <  1:406296/76‑1 (MQ=255)                  aaaaCCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGatat                                                       >  1:405725/1‑76 (MQ=255)                         ggCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGt                                                <  1:169428/76‑1 (MQ=255)                          gCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTg                                               <  2:358174/76‑1 (MQ=255)                          gCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATAANTTTGGTg                                               <  2:677706/76‑1 (MQ=255)                            tCGTATGCGTAAGCAGGACATTATTCTTGCCATCCTGAANCAGCACACAAAGAGTNGCGAAGATATCTTTGGTGAt                                             >  2:832352/1‑76 (MQ=255)                             cGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATg                                            >  2:164579/1‑76 (MQ=255)                                aTGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCg                                         >  1:751447/1‑76 (MQ=255)                                 tGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGt                                        <  1:582139/76‑1 (MQ=255)                                  gCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTa                                       <  2:155595/76‑1 (MQ=255)                                   catAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTAc                                      >  2:813333/3‑76 (MQ=255)                                   cGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTAc                                      <  1:419094/76‑1 (MQ=255)                                     tAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTg                                    >  1:422679/1‑76 (MQ=255)                                          aGGACATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGat                               >  1:197471/1‑76 (MQ=255)                                             aCATTATTTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATAtt                            <  1:31735/76‑1 (MQ=255)                                              cATTATTTTTGCCATCCTGACGCAGCACACAAAGATTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTg                           <  2:669584/76‑1 (MQ=255)                                                  attTTTGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAgg                       <  2:488427/76‑1 (MQ=255)                                                    ttttGCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggat                     <  1:628538/76‑1 (MQ=255)                                                      ttGCCATCCTGAAGCAGNACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGAAATCGCAggatgg                   >  1:102814/1‑76 (MQ=255)                                                        gCCATCCTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAggatggat                 >  2:102937/1‑76 (MQ=255)                                                             ccTGAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGt            >  1:16637/1‑76 (MQ=255)                                                                gAAGCAGCACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATAGTGCAGGATGGATTTGGTTtc         >  2:670549/1‑76 (MQ=255)                                                                  agcagcACACAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTtcct       >  2:649967/1‑76 (MQ=255)                                                                       cacacaAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGtt  <  2:296639/76‑1 (MQ=255)                                                                          |                                                                         CGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTT  >  REL606/3928270‑3928415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑