| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,071,973 | C→T | A166T (GCT→ACT) | torS ← | hybrid sensory histidine kinase in two‑component regulatory system with TorR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,071,973 | 0 | C | T | 93.8% | 48.7 / ‑2.7 | 16 | A166T (GCT→ACT) | torS | hybrid sensory histidine kinase in two‑component regulatory system with TorR |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (7/8); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.45e-01 | |||||||||||
TTTCAGCAGCCTGACGCTGATCTTGTTCGATCAAATCGTAAATCCCGGCCTGGGTCGCTCCAGCGGAAGTTGTCGCATTATTCGCCTGACCTTGCGCCAGGCGTGCGATCTCATCGGCGGCAGCGACTATCTGCT > REL606/1071910‑1072044 | tttCAGCAGCCTGACGCTGATCTTGTTCGATCAAATCGTAAATCCCGGCCTGGGTCGCTCCAGTGGAAGTTGTCGc < 1:134836/76‑1 (MQ=255) agcCTGACGCTGATCTTGTTCGATCAAATCGTAAATCCCGGCCTGGGTCGCTCCAGTGGAAGTTGTCGCATTATTc < 2:624432/76‑1 (MQ=255) ccTGACGCTGATCTTGTTCGATCAAATCGTAAATCCCGGCCTGGGTCGCTCCAGTGGAAGTTGTCGCATTATTCGc < 1:32225/76‑1 (MQ=255) cGCTGATCTTGTTCGATCAAATCGTAAATCCCGGCCTGGGTCGCTCCAGTGGAAGTTGTCGCATTATTCGCCTGAc < 2:230209/76‑1 (MQ=255) gCTGATCTTGTTCGATCAAATCGTAAATCCCGGCCTGGGTCGCTCCAGTGGAAGTTGTCGCATTATTCGCCTGAcc > 2:452875/1‑76 (MQ=255) tCTTGTTCGATCAAATCGTAAATCCCGGCCTGGGTCGCTCCAGTGGAAGTTGTCGCATTATTCGCCTGACCTTgcg < 2:649754/76‑1 (MQ=255) ttGTTCGATCAAATCGTAAATCCCGGCCTGGGTCGCTCCAGTGGAAGTTGTCGCATTATTCGCCTGACCTTGCGcc > 2:541967/1‑76 (MQ=255) tCGATCAAATCGTAAATCCCGGCCTGGGTCGCTCCAGTGGAAGTTGTCGCATTATTCGCCTGACCTTGCGCCAGGc > 2:667170/1‑76 (MQ=255) cGTAAATCCCGGCCTGGGTCGCTCCAGTGGAAGTTGTCGCATTATTCGCCTGACCTTGCGCCAGGCGTGCGAtctc < 2:776205/76‑1 (MQ=255) aaaTCCCGGCCTGGGTCGCTCCAGTGGAAGTTGTCGCATTATTCGCCTGACCTTGCGCCAGGCGTGCGATCTCATc > 1:773515/1‑76 (MQ=255) aaTCCCGGCCTGGGTCGCTCCAGCGGAAGTTGTCGCATTATTCGCCTGACCTTGCGCCAGGCGTGCGATCTCATcg < 1:262017/76‑1 (MQ=255) ccGGCCTGGGTCGCTCCAGTGGAAGTTGTCGCATTATTCGCCTGACCTTGCGCCAGGCGTGCGATCTCATcggcgg > 2:365205/1‑76 (MQ=255) cGGCCTGGGTCGCTCCAGTGGAAGTTGTCGCATTATTCGCCTGACCTTGCGCCAGGCGTGCGATCTCATCggcggc < 2:148856/76‑1 (MQ=255) cTGGGTCGCTCCAGTGGAAGTTGTCGCATTATTCGCCTGACCTTGCGCCAGGCGCGCGATCTCATCGGCGGCACCg > 2:231318/1‑76 (MQ=255) gCTCCAGTGGAAGTTGTCGCATTATTCGCCTGACCTTGCGCCAGGCGTGCGATCTCATCGGCGGCAGCGACTATct < 2:600748/76‑1 (MQ=255) ccAGTGGAAGTTGTCGCATTATTCGCCTGACCTTGCGCCAGGCGTGCGATCTCATCGGCGGCAGCGACTATctgct > 2:624402/1‑76 (MQ=255) | TTTCAGCAGCCTGACGCTGATCTTGTTCGATCAAATCGTAAATCCCGGCCTGGGTCGCTCCAGCGGAAGTTGTCGCATTATTCGCCTGACCTTGCGCCAGGCGTGCGATCTCATCGGCGGCAGCGACTATCTGCT > REL606/1071910‑1072044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |