Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,305,548	T→C	D231G (GAT→GGT)	cls ←	cardiolipin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,305,548	0	T	C	100.0%	104.2 / NA	25	D231G (GAT→GGT)	cls	cardiolipin synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/12);  total (13/12)

CGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTA  >  REL606/1305480‑1305620                                                                     |                                                                         cGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTACCgatcat                                                                   <  1:499579/76‑1 (MQ=255)   cccGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTACCgatcatga                                                                 <  2:87408/76‑1 (MQ=255)          cTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTACCGATCATGATCATCtt                                                          >  1:575942/1‑76 (MQ=255)            tGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTACCgatcatgatca                                                              <  2:744228/70‑1 (MQ=255)            tGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTACCgatcatgatca                                                              >  1:744228/1‑70 (MQ=255)            tGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTACCGATCATGATCATCTTGc                                                        >  2:184578/1‑76 (MQ=255)            tGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTACCGATCATGATCATCTTGc                                                        >  1:109348/1‑76 (MQ=255)                tGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTACCGATCATGATCATCTTGCGATg                                                    <  1:558951/76‑1 (MQ=255)                   aGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTACCGATCATGATCATCTTGCGATGTTg                                                 <  2:192464/76‑1 (MQ=255)                      aGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTACCGATCATGATCATCTTGCGATGTTGgcg                                              >  1:529801/1‑76 (MQ=255)                         gAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTACCGATCATGATCATCTTGCGATGTTGGCGCAg                                           <  1:767053/76‑1 (MQ=255)                              tCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTACCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCa                                      >  1:447259/1‑76 (MQ=255)                              tCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTACCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCa                                      <  2:419767/76‑1 (MQ=255)                                 aCCATATTCATGCTGCCGGTGTACGCGATGTAATTACCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATAc                                   <  2:504655/76‑1 (MQ=255)                                    atatTCATGCTGCCGGTGTACGCGATGTAATTACCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGc                                <  1:439709/76‑1 (MQ=255)                                    atatTCATGCTGCCGGTGTACGCGATGTAATTACCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGc                                >  2:114035/1‑76 (MQ=255)                                      atTCATGCTGCCGGTGTACGCGATGTAATTACCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGt                              >  1:465535/1‑76 (MQ=255)                                      atTCATGCTGCCGGTGTACGCGATGTAATTACCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGt                              >  1:166029/1‑76 (MQ=255)                                         cATGCTGCCGGTGTACGCGATGTAATTACCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTaaa                           <  1:523653/76‑1 (MQ=255)                                                ccGGTGTACGCGATGTAATTACCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCTTAAAAacacac                    <  1:45275/76‑1 (MQ=255)                                                 cGGTGTACGCGATGTAATTACCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACg                   >  2:494654/1‑76 (MQ=255)                                                            aTGTAATTACCGATCATGATCATCTTGCGATGTTGGCCCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGa        >  1:709934/1‑76 (MQ=255)                                                              gTAATTACCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGAcc      <  1:252022/76‑1 (MQ=255)                                                                aaTTACCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCtt    >  2:549584/1‑76 (MQ=255)                                                                  ttACCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTa  >  1:415670/1‑76 (MQ=255)                                                                     |                                                                         CGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTA  >  REL606/1305480‑1305620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑