| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,329,516 | 0 | C | T | 100.0% | 30.1 / NA | 11 | H33Y (CAC→TAC) | topA | DNA topoisomerase I |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (7/4); total (7/4) | |||||||||||
TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCT > REL606/1329443‑1329587 | tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtac > 1:455016/1‑76 (MQ=255)tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtac < 1:711330/76‑1 (MQ=255) gAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtaca < 1:426941/76‑1 (MQ=255) aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc > 2:584239/1‑76 (MQ=255) gTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGcc > 2:485018/1‑76 (MQ=255) tGACTACGTGGTGAAATCCAGCGTCGGNTACATCCGTGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGcc > 1:735664/1‑76 (MQ=255) gACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGGGGCTCAACTGCCGAAAAGAGTGCCg > 1:414683/1‑76 (MQ=255) cTACGTGGTGAAATCCAGCGGCGGTTACATCCGCGCTTTACCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAc < 2:675723/76‑1 (MQ=255) tACGTGATGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAct > 1:353483/1‑76 (MQ=255) gtggtaAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa > 1:50695/1‑76 (MQ=255) gtgAAATCCAGNATCGGTTACATCCGCGATTTGCCGCCCCGTGGNTCAGCTGCCAAAAAGAGTGCCCACTCTACCt < 1:98269/76‑1 (MQ=255) aTNCTGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTctncc < 2:685446/76‑1 (MQ=255) aTCCAGCGTTGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacc < 2:296673/76‑1 (MQ=255) aTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacc < 2:175149/76‑1 (MQ=255) ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa > 1:376022/1‑76 (MQ=255) cGGTTACATCCGCGATTTGCCGACGAGTGGCTCAGCTGCCAAAAAGAGTGCCGTCTGTTCCCCCACCAAGACGGCt > 1:41829/1‑76 (MQ=255) | TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCT > REL606/1329443‑1329587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |