Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	59.4 / NA	21	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/8);  total (13/8)

ATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGC  >  REL606/2193710‑2193846                                                                      |                                                                    aTGCATCCGGCGTCCGGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccac                                                               <  2:331519/76‑1 (MQ=255)            gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa                                                    >  2:756346/1‑76 (MQ=255)             gccAGGCGCGGCTGGTCTGGCTGTCTGAGATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt                                                   <  1:130135/75‑1 (MQ=255)              ccAGGCTCGGNTGGTCTGGCTNTCGGAAATCAGCGCCAATAACAGCAAAACGCCAATCCCCAACAGATAAAGCATg                                                  >  2:825453/1‑76 (MQ=255)              ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCGAATCACAGCAAAACGCCAGTCCCCACCAGATAAAGCATg                                                  >  2:667892/1‑76 (MQ=255)              ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                  >  2:39060/1‑76 (MQ=255)                      ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                          >  1:256584/1‑76 (MQ=255)                       gCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                         <  2:104553/76‑1 (MQ=255)                             cTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                   >  2:185550/1‑76 (MQ=255)                                 cTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                               >  2:370921/1‑76 (MQ=255)                                   gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATTAAGCATGACGCCTGTATTAAACAGGCtt                             <  2:604009/76‑1 (MQ=255)                                   gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                             >  2:601857/1‑76 (MQ=255)                                     cGGAAATCAGCCCCAATAACAGCAAACCGCCACTCCCCACCAGATAAAGCATGACGCCTGTATCGAACAGGCtttt                           >  1:144008/1‑76 (MQ=255)                                     cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                           <  2:305553/76‑1 (MQ=255)                                     cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                           >  1:144013/1‑76 (MQ=255)                                          aTNAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCCTTTTGNCg                      <  2:688967/76‑1 (MQ=255)                                           tCAGCCCCAATAACAGCAAAACGCCAATCCCCACNAGATCAANGANGCNGCCTGTATGAAACAGGCTTTTTACCGa                     >  1:733493/1‑76 (MQ=255)                                                cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                <  1:767766/76‑1 (MQ=255)                                                      aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc          >  1:412417/1‑76 (MQ=255)                                                      aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc          >  2:807506/1‑76 (MQ=255)                                                              aaCACCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc  <  1:605232/76‑1 (MQ=255)                                                                      |                                                                    ATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGC  >  REL606/2193710‑2193846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑