| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 475,115 | C→A | G64V (GGC→GTC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 475,115 | 0 | C | A | 88.9% | 110.2 / 4.4 | 36 | G64V (GGC→GTC) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base C (1/3); new base A (19/13); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
GGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACAC > REL606/475042‑475187 | ggCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGacc > 1:959638/1‑76 (MQ=255) cGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGACCAg > 2:1249142/1‑76 (MQ=255) cGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGACCAg > 1:550921/1‑76 (MQ=255) tGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGACCAGTTc < 2:1036324/76‑1 (MQ=255) ggCCTTTAGCACCGTCNATTCCTTCAGCGAGANGTGCAAATCGACGCCAAACATCAACAGAATGANGACCAGNTNa > 1:116160/1‑76 (MQ=255) gCCTTTACCGCCATCAAGTCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAg < 1:169219/76‑1 (MQ=255) gCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGAAGCCCAGTTCAg < 1:1305872/76‑1 (MQ=255) ccTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGACCAGTTCAGc < 1:284227/76‑1 (MQ=255) cTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCGCAGTTCAGcc > 2:779987/1‑76 (MQ=255) cTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGACCATTTCAGcc < 2:1569624/76‑1 (MQ=255) cGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTcc > 1:520148/1‑76 (MQ=255) aTCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTCCgggg > 2:109542/1‑76 (MQ=255) tCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGc > 1:378520/1‑76 (MQ=255) ccTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCtt < 2:13742/76‑1 (MQ=255) cTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTg > 1:229362/1‑76 (MQ=255) tCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGt < 2:862456/76‑1 (MQ=255) aGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTAt > 2:1123375/1‑76 (MQ=255) aaGTGCAAACCGACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGcaa > 1:744079/1‑76 (MQ=255) gTGCAAACCGACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGcaaca < 1:1401473/76‑1 (MQ=255) gCAAACCGACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACaaa < 2:1092137/76‑1 (MQ=255) gCAAACCGACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACaaa < 1:933582/76‑1 (MQ=255) cAAACCGACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAg > 2:646837/1‑76 (MQ=255) cAAACCGACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAg < 2:468689/76‑1 (MQ=255) cAAACCGACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAg < 2:439593/76‑1 (MQ=255) aacccgacgccgacgATCAGCAGAGTGGCGCCGAGTTGAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGc < 1:272082/73‑1 (MQ=255) aaaCCGACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGc > 2:1154516/1‑76 (MQ=255) gACGCCAAACATCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGa > 1:447717/1‑76 (MQ=255) cGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGt < 1:459725/76‑1 (MQ=255) aaaCATCAACAGAATGACGACCAGTTCAGCGAGTTCCGGGGCAAGCTTGGTATCGGCAACAANGCCCGGAGTGAAt > 2:1476663/1‑76 (MQ=255) aaaCATCAAAAGAATGACGACCAGTTCAGCCAGCTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAAt > 2:1394295/1‑76 (MQ=255) aaCATCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAANCCCGGAGTGAATg > 1:212035/1‑76 (MQ=255) aTCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTc > 2:1261133/1‑76 (MQ=255) aTCAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTc < 2:1000502/76‑1 (MQ=255) cAACAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCt < 1:299055/76‑1 (MQ=255) aaCAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTg < 2:116160/76‑1 (MQ=255) aCAGAATGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGc > 2:712478/1‑76 (MQ=255) aTGACGACCAGTTCAGCCAGTTCCGGGNCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGca > 1:1616508/1‑76 (MQ=255) tGACGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCGGCCAGcac < 1:807472/76‑1 (MQ=255) gacgacCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGcaca < 1:1114802/76‑1 (MQ=255) aCGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCacac > 2:1551905/1‑76 (MQ=255) aCGACCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCacac > 1:763084/1‑76 (MQ=255) | GGCGATGGCCTTTACCGCCATCAAATCCTTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACAC > REL606/475042‑475187 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |