Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	176.4 / NA	56	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/38);  total (18/38)

TGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAG  >  REL606/2193711‑2193845                                                                     |                                                                   tGCATCCGGCGTCCAGGCNCGGCTGGTCTGGCTGTCGGAAATCAGCTCCAATAACAGCAAAACGCCAATCCccacc                                                             >  1:1542857/1‑76 (MQ=255)  gCATCCGGCCTCCAGGCGCGGCTGGTCTGGCTGCCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca                                                            >  2:1390057/1‑76 (MQ=255)     tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGGAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                         >  1:608959/1‑76 (MQ=255)     tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                         <  1:1037450/76‑1 (MQ=255)     tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                         <  2:827870/76‑1 (MQ=255)      ccGGCGTCCAGGCNCGGCTGGTCTGGCTNTCNGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                                                        <  2:1459123/76‑1 (MQ=255)       cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                       <  2:62582/76‑1 (MQ=255)        ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATGACAGCAAAACGCCAATCCCCACCAGATaaa                                                      <  1:1479182/76‑1 (MQ=255)        ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                      >  1:1412828/1‑76 (MQ=255)          cGTCCAGGCACGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc                                                    <  1:54555/76‑1 (MQ=255)           gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCCCCAGATAAAGCa                                                   >  2:588884/1‑76 (MQ=255)           gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa                                                   >  2:175930/1‑76 (MQ=255)             ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAATACGCCAATCCCCACCAGATAAAGCATg                                                 <  1:1118677/76‑1 (MQ=255)             ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                 <  2:113176/76‑1 (MQ=255)             ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                 <  1:1173771/76‑1 (MQ=255)             ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                 <  1:1232093/76‑1 (MQ=255)                     ngCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATANAGCATGACGCCTGt                                         >  2:219988/2‑76 (MQ=255)                     ggCTGGTCGGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                         <  1:1438255/76‑1 (MQ=255)                      gCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACCCCTGTa                                        >  2:403804/1‑76 (MQ=255)                       cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                       >  1:573803/1‑76 (MQ=255)                       cTGGTCTGGCTGTCGGAAATCAGCCCCAAGAACAGCCAAACGCCAATCCCCACCAGANAAAGCATGACGCCTGTAt                                       <  1:1543751/76‑1 (MQ=255)                        tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                      <  2:458865/76‑1 (MQ=255)                        tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                      <  2:732808/76‑1 (MQ=255)                         ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                     <  2:552880/76‑1 (MQ=255)                         ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                     >  2:1412630/1‑76 (MQ=255)                         ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                     <  1:495303/76‑1 (MQ=255)                           tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                   <  1:1359732/76‑1 (MQ=255)                           tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                   <  2:267801/76‑1 (MQ=255)                            cTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                  >  1:812749/1‑76 (MQ=255)                             tGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTNTATTAAACa                                 <  1:36194/76‑1 (MQ=255)                             tGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                 <  1:859322/76‑1 (MQ=255)                              ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                <  2:721557/76‑1 (MQ=255)                              ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                <  1:1559879/76‑1 (MQ=255)                                  gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAGAGGCtt                            <  2:344777/76‑1 (MQ=255)                                  gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                            <  2:1181499/76‑1 (MQ=255)                                  gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                            >  2:307563/1‑76 (MQ=255)                                  gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                            >  1:365676/1‑76 (MQ=255)                                   tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                           <  2:280031/76‑1 (MQ=255)                                     ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                         <  1:1401706/76‑1 (MQ=255)                                     ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                         <  1:718316/76‑1 (MQ=255)                                     ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                         <  2:45196/76‑1 (MQ=255)                                     ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttct                         <  1:417366/76‑3 (MQ=255)                                        aaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                      >  1:1064336/1‑76 (MQ=255)                                            aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                  <  2:790342/76‑1 (MQ=255)                                            aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                  <  2:626921/76‑1 (MQ=255)                                             gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                 <  2:889258/76‑1 (MQ=255)                                             gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                 <  2:375936/76‑1 (MQ=255)                                             gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                 <  1:1028383/76‑1 (MQ=255)                                              ccccAATAAGAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTTCCGATGgc                <  1:57001/76‑1 (MQ=255)                                              ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAANCAGGCTTTTTACCGATGgc                <  1:1503265/76‑1 (MQ=255)                                                ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc              >  2:1113701/1‑76 (MQ=255)                                                ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATCAAACAGGCTTNTTACCGATggtca              >  1:211764/1‑73 (MQ=255)                                                    tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg          <  2:1497407/76‑1 (MQ=255)                                                     aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc         <  1:158344/76‑1 (MQ=255)                                                          cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc    >  2:140855/1‑76 (MQ=255)                                                          cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc    >  2:1027945/1‑76 (MQ=255)                                                            aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg  <  1:794010/76‑1 (MQ=255)                                                                     |                                                                   TGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAG  >  REL606/2193711‑2193845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑