| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,145,998 | C→T | I45I (ATC→ATT) | rpoD → | RNA polymerase sigma factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,145,998 | 0 | C | T | 98.4% | 244.3 / ‑3.6 | 64 | I45I (ATC→ATT) | rpoD | RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (33/30); total (33/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
TCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGA > REL606/3145926‑3146069 | tCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCNGATCGAAGACATCATTCaa < 1:1506966/76‑1 (MQ=255)tCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCaa < 1:64274/76‑1 (MQ=255)tCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCaa > 1:1359264/1‑76 (MQ=255) cTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGTCATCATTCaaa > 1:217671/1‑76 (MQ=255) tGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAAt > 1:1171241/1‑76 (MQ=255) gACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATg > 1:662604/1‑76 (MQ=255) tcTATGCCGAGGTCAATGACCATCTGCGGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGAt < 1:274064/75‑1 (MQ=255) ccTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGAt > 2:1438310/1‑76 (MQ=255) ccTATGCCGAGGTCAATGACCATCTGCCGGAAGATAGCGTCGATTCAGATCAGATCGNAGACATCATTCAAATGAt < 1:1545282/76‑1 (MQ=255) cTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATc < 2:637675/76‑1 (MQ=255) tATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCa < 2:126815/76‑1 (MQ=255) tATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCa < 2:1404266/76‑1 (MQ=255) aTGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCaa < 1:556742/76‑1 (MQ=255) aTGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCaa < 2:1011505/76‑1 (MQ=255) gCCGAGGTCAATGNCCATCTGCCGGAAGATATCGTCGATTCAGATTAGATCGAAGACATCATTCAAATGATCAACg < 2:1483085/76‑1 (MQ=255) gCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACg < 2:77984/76‑1 (MQ=255) ccGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGa < 2:1219348/76‑1 (MQ=255) gAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACa > 2:1142707/1‑76 (MQ=255) gTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATgg < 1:1176338/76‑1 (MQ=255) cAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGc > 2:868391/1‑76 (MQ=255) cAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGc > 2:531216/1‑76 (MQ=255) cAATGACCATCTGCCGGAAGAAATGGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGc < 1:1518437/76‑1 (MQ=255) aaTGACCATCTGCCGGAAGATATCGTCGATTCANTTAAGATCGAAGACATCATTCAAATGATCAACGACATGGGCa > 2:1377354/1‑76 (MQ=255) tGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCAtt < 2:298789/76‑1 (MQ=255) tGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCAtc < 2:1490871/76‑2 (MQ=255) aCCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCa < 1:1260231/76‑1 (MQ=255) ccATCTGCNGGAAGATATCGTCGATTCAGATCAGATCGATGACATCATTCAAATGATCAACGACATGGGCATTCAg < 1:1428528/76‑1 (MQ=255) ccATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAg < 1:428464/76‑1 (MQ=255) aTCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGt > 1:842337/1‑76 (MQ=255) cTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGa > 2:1121345/1‑76 (MQ=255) cTGCCGGAAAATATCGTCGATTCNGATCANATCGAGGACATCATNCAGATGCTCAACGACACGGGCATTCAGGTGa > 2:201054/1‑76 (MQ=255) gCCGGAAGATATTGTCGATTCAGATCAGATCGNAGCCATCANTCAAAGGATCAACGACATGNGCATTCAGGTGATg > 2:1331545/1‑76 (MQ=255) gCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATg > 2:78570/1‑76 (MQ=255) ccGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATgg > 2:704570/1‑76 (MQ=255) cGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGga < 1:868391/76‑1 (MQ=255) ggAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGgaa > 2:765921/1‑76 (MQ=255) ggAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGgaa < 1:1240031/76‑1 (MQ=255) gAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGgaag > 1:277571/1‑76 (MQ=255) gAAGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATGCAGGTGATGgaag < 1:114533/76‑1 (MQ=255) aaGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGgaaga < 2:1138046/76‑1 (MQ=255) aGATATCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGgaagaa > 1:613707/1‑76 (MQ=255) atCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCAc > 1:1219451/1‑76 (MQ=255) atCGTCGATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCAc > 1:1180016/1‑76 (MQ=255) cgtcaATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCg > 2:1594402/1‑76 (MQ=255) cgATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGAt < 1:1194641/76‑1 (MQ=255) cgATTCAGACCAGATCGAAGACANCATTCNAATGACCAACGACANGGGCATACAGGTGATGAAAGAAGCACCGGAt > 2:210169/1‑76 (MQ=255) gATTCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATg < 2:1609536/76‑1 (MQ=255) ttCAGATCAGATCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGcc > 2:1494434/1‑76 (MQ=255) ttCAGATCAGATCGAAGACATCATTCAAATGANCATCGACANGGGCAATCAGGTGATGGAANAAGCACCGGATGcc > 2:1331888/1‑76 (MQ=255) tcagatcagaTCGNAGACATCATTCAAANGANCAACGACATGGGCATTGAGGTGATGGAAGAAGCACCGGATGCCg < 2:1461827/76‑1 (MQ=255) tcagatcagaTCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCg > 1:478177/1‑76 (MQ=255) cagatcagatCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCga < 2:169255/76‑1 (MQ=255) cagatcagatCGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCga > 2:32103/1‑76 (MQ=255) atcagatcGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCgatga > 1:984949/1‑76 (MQ=255) tcagaacGAAGACATCATTCAAATGCTCAACAATATGGGCATTCAGGTGATGGAAGAAGCGCCGGATGCCgatgat < 2:1584388/76‑1 (MQ=255) atcGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGa > 2:461693/1‑76 (MQ=255) tcGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGAt > 2:41269/1‑76 (MQ=255) tcGAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGAt < 2:740092/76‑1 (MQ=255) gAAGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATgc > 1:1268262/1‑76 (MQ=255) aaGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATgct < 2:476162/76‑1 (MQ=255) aaGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATgct > 2:111905/1‑76 (MQ=255) aaGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATgat > 1:879510/1‑74 (MQ=255) aaGACATCATTCAAATGATCAAAGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATgct > 2:463109/1‑76 (MQ=255) ataCATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATgctg > 1:452087/3‑76 (MQ=255) aGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATgctg < 2:682639/76‑1 (MQ=255) aGACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATgctg > 1:1196888/1‑76 (MQ=255) gACATCATTCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATgctgg < 2:403063/76‑1 (MQ=255) tcattCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGa < 2:608409/76‑1 (MQ=255) | TCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGCATTCAGGTGATGGAAGAAGCACCGGATGCCGATGATCTGATGCTGGCTGA > REL606/3145926‑3146069 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |