Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,190,370	A→G	L204P (CTG→CCG)	yhaO ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,190,370	0	A	G	100.0%	140.0 / NA	35	L204P (CTG→CCG)	yhaO	predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/22);  total (13/22)

TTCCCGCGAGCGATAAGAGATCACCATCGGGCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATT  >  REL606/3190297‑3190441                                                                          |                                                                        ttCCCGCGAGCGATAAGAGATCACCATCGGGCTTAACGTCTGAATAAACAGAATCGACGCCAGGGTAAACggcggc                                                                       >  2:1582954/1‑76 (MQ=255)  tCCCGCGANCGATAAGAGATCACCATCGGGCTTAACGTCAGAATAAACAGAATCGACGTCAGGGTAAACGgcggcg                                                                      <  1:1428334/76‑1 (MQ=255)       cgagcgaTAAGAGATCACCATCGGGCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAGCGGCGGCGtaata                                                                 <  1:109662/76‑1 (MQ=255)        gagcgaTAAGAGATCACCATCGGGCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGtaataa                                                                >  2:1557890/1‑76 (MQ=255)             aTAAGAGGTCACCATCGGGCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCg                                                           <  2:628749/76‑1 (MQ=255)               nagagATCACCATCGGGCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGNGTAATAATGGCGtt                                                         >  2:218209/2‑76 (MQ=255)               aaGAGATCACCATCGGGCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGtt                                                         >  2:1145110/1‑76 (MQ=255)               aaGAGATCACCATCGGGCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGtt                                                         >  1:767208/1‑76 (MQ=255)                   gaTCACCATCGGGNTTAACGTCTGAATANACNTAATCGACGTCAGGGTCAACGGCGGCGTAATAATGGCGTTTTTc                                                     <  2:1457775/76‑1 (MQ=255)                      cACCATCGGGCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCAcc                                                  <  1:1160591/76‑1 (MQ=255)                       aCCATCGGGCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACca                                                 <  2:1179311/76‑1 (MQ=255)                        ccATCGGGCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACcag                                                <  1:394283/76‑1 (MQ=255)                           tCGGGCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAGTAATGGCGTTTTTCACcagcag                                             <  1:1173050/76‑1 (MQ=255)                           tCGGGCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACcagcag                                             <  2:889523/76‑1 (MQ=255)                           tCGGGCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACcagcag                                             <  2:86289/76‑1 (MQ=255)                              ggCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACCAGCAGccc                                          >  1:822140/1‑76 (MQ=255)                              ggCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACCAGCAGccc                                          <  2:167505/76‑1 (MQ=255)                                 ttAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACCAGTAGCCCCAg                                       >  1:585527/1‑76 (MQ=255)                                 ttAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAg                                       <  2:1013109/76‑1 (MQ=255)                                 ttAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAg                                       >  2:1494851/1‑76 (MQ=255)                                  tAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAgc                                      <  2:373598/76‑1 (MQ=255)                                   aaCGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAgcg                                     <  1:1570210/76‑1 (MQ=255)                                       tCTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGcggcgg                                 <  2:1284195/76‑1 (MQ=255)                                        cTGAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGt                                <  2:146611/76‑1 (MQ=255)                                          gAATAAACNGCATCGACGTNAGGGTAAACGGCGGCGTAATAANGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAg                              <  2:1355470/76‑1 (MQ=255)                                          gAATAAACAGAATCGACGTCGGNNTAAACGGCGGNNAAATAATGACGTTTCTCACCAGCAGCCCCAGCGGCGGTAg                              <  1:1462704/76‑1 (MQ=255)                                          gAATAAACAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAg                              <  2:155541/76‑1 (MQ=255)                                              aaaCAGAATCGACGTCATGGTATACGGCGGCGTAATAATGGCGTTTTTCACCAGAAGCCCCTGCGGCGGTAGCGaa                          >  2:239692/1‑76 (MQ=255)                                               aaCAGAATCGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAAc                         <  2:1240101/76‑1 (MQ=255)                                                       cGACGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTta                 >  1:155053/1‑76 (MQ=255)                                                        gACGTCAGGGTAAACGGTGGCGTAATATTGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTtat                >  1:85188/1‑76 (MQ=255)                                                          cGTCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATAc              >  1:661746/1‑76 (MQ=255)                                                           gTCAGGGTAAACGNCGGCGTAATAATGGCGATTTTCACCAGCAGCTCCAGCGGCGGTAGCGAACCGACGTTATACa             <  2:1475516/76‑1 (MQ=255)                                                            tCAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAg            <  1:934319/76‑1 (MQ=255)                                                             cAGGGTAAACGGCGGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGGAGCGAACCGACGTTATACAGa           >  1:1190014/1‑76 (MQ=255)                                                                   aaaCGGCGGCGTAATAATGGCGTTTTTCACCCGCAGCCCCNNCGGCGGTAGCNNAGCGACGTTATACAGATGCcac     >  1:1454512/1‑76 (MQ=255)                                                                     aCGGCGGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACAt   <  1:891957/76‑1 (MQ=255)                                                                      cggcggCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACAtt  >  2:477685/1‑76 (MQ=255)                                                                          |                                                                        TTCCCGCGAGCGATAAGAGATCACCATCGGGCTTAACGTCTGAATAAACAGAATCGACGTCAGGGTAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATT  >  REL606/3190297‑3190441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑