| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,108,753 | C→T | G254S (GGC→AGC) | ECB_01996 ← | baseplate assembly protein J |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,108,753 | 0 | C | T | 94.7% | 51.4 / ‑5.5 | 19 | G254S (GGC→AGC) | ECB_01996 | baseplate assembly protein J |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (10/8); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
AGAAAGACGCCTGCGTGCTGTTAAGCACGATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCCTTCCACATGCAAAGCGGCATAAATGGCAGACAGACGGATGTCGCGCCCCAGCCGGTGCTGTGCCGTGATGT > REL606/2108684‑2108824 | aGAAAGACGCCTGCGTGCTGTTAAGCACGATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCTTTcccc > 1:348755/1‑74 (MQ=255) aaaGACGCCTGCGTGCTGTTAAGCACGATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCTTTCCACAt > 1:181572/1‑76 (MQ=255) aaGACGCCTGCGTGCTGTTAAGCACGATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCTTTCCACATg > 1:308162/1‑76 (MQ=255) gACGCCTGCGTGCTGTCAAGCACGATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCTTTCCACATGCa < 2:512298/76‑1 (MQ=255) gCCTGCGTGCTGTTAAGCACGATATCGGCCAGCGGTGCAGCCAGTTCGACCCGCTGCACGCTTTCCACATGcgca > 2:673372/1‑72 (MQ=255) gCCTGCGTGCTGTTAAGCACGATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCTTTCCACATGCaca < 1:673372/75‑3 (MQ=255) ccTGCGTGCTGTTAAGCACGATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCTTTCCACATGCAAAGc < 1:555577/76‑1 (MQ=255) cGTGCTGTTAAGCACGATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCTTTCCACATGCAAAGCGGCa > 2:423111/1‑76 (MQ=255) gTTAAGCACGATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCTTTCCACATGCAAAGAGGCATaaaag > 1:98660/1‑74 (MQ=255) ttAAGCACGATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCTTTCCACATGCAAAGCGGCATAAATgg > 2:323363/1‑76 (MQ=255) tAAGCACGATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCTTTCCACATGCAAAGCGGCATAAATGGc < 1:162556/76‑1 (MQ=255) tAAGCACGATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCTTTCCACATGCAAAGCGGCATAAATGGc < 2:385460/76‑1 (MQ=255) aCGATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCTTTCCACATGCAAAGCGGCATAAATGGcagaca < 1:427277/76‑1 (MQ=255) gATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCTTTCCACATGCAAAGCGGCCTAAATGGcagacaga > 2:356032/1‑76 (MQ=255) gATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCTTTCCACATGCAAAGCGGCATAAATGGcagacaga < 2:740011/76‑1 (MQ=255) gCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCTTTCCACATGCAAAGCGGCATAAATGGCAGACAGACGGATGt > 2:471431/1‑76 (MQ=255) cggCGGTGCAGCCAGTTCGGCACGCGGCACGCTTTCCACATGCAAAGCGGCATAAATGGCAGACAGACGGATGTcg < 2:215128/74‑1 (MQ=255) gtcGGTGCAGCCAGTTCGACACGCTGCACGCTTTCCACATGCAAAGCGGCATAATTGGCAGACAGACGGATGTcgc > 2:656173/3‑76 (MQ=255) tGCAGCCAGTTCGACACGCTGCACGCTTTCCACATGCAAAGCGGCATAAATGGCAGACAGACGGATGTCGCGcccc > 1:406334/1‑76 (MQ=255) aGTCCGCCACGCTGCACGCTTTCCACATGCAAAGCGGCATAAATGGCAGACAGACGGATGTCGCGCCCCAGCCGGt < 2:70080/76‑1 (MQ=255) cTGCCCGCTTCCCACATGCAAAGCGGCATAAATGGCAGACAGCGGGATGCCGCGCCCCAGCCGGTGCTGTGCCGTg < 1:423111/76‑1 (MQ=255) gCACGCTTTCCACATGCAAAGCGGCATAAATAGCAGACAGACGGATGTCGCGCCCCAGCCGGTGCTGTGCCGTGAt > 2:625489/1‑76 (MQ=255) aCGCCTTCCACATGCAAAGCGGCATAAATGGCAGACAGACGGATGTCGCGCCCCAGCCGGTGCTGTGCCGTGATGt < 2:495783/76‑1 (MQ=255) | AGAAAGACGCCTGCGTGCTGTTAAGCACGATATCGGCCAGCGGTGCAGCCAGTTCGACACGCTGCACGCCTTCCACATGCAAAGCGGCATAAATGGCAGACAGACGGATGTCGCGCCCCAGCCGGTGCTGTGCCGTGATGT > REL606/2108684‑2108824 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |