| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,193,779 | 0 | G | T | 95.2% | 57.0 / NA | 21 | L143I (CTT→ATT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (5/15); minor base C (1/0); total (6/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACC > REL606/2193707‑2193843 | ccGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATccc > 2:578259/1‑76 (MQ=255) cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc > 2:256296/1‑76 (MQ=255) ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa < 1:441153/76‑1 (MQ=255) gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa > 1:172479/1‑76 (MQ=255) cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa < 1:193593/76‑1 (MQ=255) cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa > 2:268802/1‑76 (MQ=255) aGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc < 2:226270/76‑1 (MQ=255) ggCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg < 2:427316/76‑1 (MQ=255) gcgcGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGc < 1:751840/76‑1 (MQ=255) gcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAGTCCCCACCAGATAAAGCATGACGCCt < 2:95819/76‑1 (MQ=255) gacTGGTCTGGCTGTCGGAAAACAGCCCCGATGACAGCAATACGCCAAACCCCACNAGATAAAGCATAACGCCTGt < 1:92358/74‑1 (MQ=255) tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt < 1:268769/76‑1 (MQ=255) tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa < 1:31796/76‑1 (MQ=255) tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAACCCCCACCAGATAAAGCATGACGCCTGTATTaaa > 1:623442/1‑76 (MQ=255) ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg < 2:172479/76‑1 (MQ=255) gAAATCAGCCCCATTAACAGCAAAACGTCAATCCCCACCAGGTAAAGCATGACGCCTGTATTAAACACCCTTTTTa < 2:701343/76‑1 (MQ=255) gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATTNAGCATGACGTCTGTATTAAACAGGCTTTTTa < 1:71408/76‑1 (MQ=255) gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa < 2:177078/76‑1 (MQ=255) aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCATATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg < 1:551078/76‑1 (MQ=255) ccccAATAACAGCAAAACGCCAATCCCCACCAGTTAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc < 1:625951/76‑1 (MQ=255) cacAATCACAGCAAAACGCCAANCCTCACCAGANAAAGCATGNCGCCTGTATTAAACAGGCGTTTNACCGTTGgcg < 2:682185/74‑1 (MQ=255) tAACAGCAAAACGGCAATCCTCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg < 2:660687/76‑1 (MQ=255) cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc > 2:169667/1‑76 (MQ=255) | CCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACC > REL606/2193707‑2193843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |