Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,409,177	G→A	G91G (GGC→GGT)	vacJ ←	predicted lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,409,177	0	G	A	100.0%	79.3 / NA	22	G91G (GGC→GGT)	vacJ	predicted lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/14);  total (8/14)

AACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGCCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGC  >  REL606/2409110‑2409249                                                                    |                                                                         naCCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCaa                                                                  <  1:673345/75‑1 (MQ=255)  aCCGCGCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCaag                                                                 <  2:620815/76‑1 (MQ=255)        cATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAagtag                                                           >  1:338833/1‑76 (MQ=255)           ccccAAAATGGTGTTTAGGAAAAAGAGGGAAAAGTGGACAATCCCCTGATAAGGGTGACCCTGCAAGTAGTAGTTa                                                        >  1:417935/1‑76 (MQ=255)             ccAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGCAGTTAAc                                                      <  1:491642/76‑1 (MQ=255)              cAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAAcc                                                     >  2:111028/1‑76 (MQ=255)               aaaaTGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCa                                                    >  2:539390/1‑76 (MQ=255)                 aaTGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATc                                                  >  2:501338/1‑76 (MQ=255)                               aaaaGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGttcttc                                    <  2:757253/76‑1 (MQ=255)                                 aaGCGGGTAAAGTGGACCTTCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCaa                                  >  2:711764/1‑76 (MQ=255)                                  aGCGGGTAAAGTGGACCATCTCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAg                                 >  1:548569/1‑76 (MQ=255)                                  aGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAg                                 <  1:501338/76‑1 (MQ=255)                                  aGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAg                                 >  1:290448/1‑76 (MQ=255)                                     gggTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGtt                              <  1:539390/76‑1 (MQ=255)                                     gggTAAAGTGGACCATCCCCTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGtt                              <  2:402769/76‑1 (MQ=255)                                             nggNCNACCCCCTTNTACGGGGTGCCGTGCAANAAGTAGTTCACCGTCACCGCAGGTTCCTCAAGGTTGCCAGTaa                      <  1:664751/75‑1 (MQ=255)                                                aCCATCCCCTGATAAGGGTCACCCTGCAAGTAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGt                   <  1:475162/76‑1 (MQ=255)                                                       ccTGATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCaa            >  1:419598/1‑76 (MQ=255)                                                          gATAAGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCAAAcc         <  1:333765/76‑1 (MQ=255)                                                             aaGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCAAACCGtt      <  2:460610/76‑1 (MQ=255)                                                              aGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCAAACCGTTa     <  2:349033/76‑1 (MQ=255)                                                              aGGGTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCAAACCGTTa     <  2:74558/76‑1 (MQ=255)                                                                ggTCACCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCACAAACCGTTAcg   <  2:6155/76‑1 (MQ=255)                                                                 gTCACCCTGCAAGAAGTAGTTAACCATCACCGCTGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCAAACGGTTAcgc  <  1:517079/76‑1 (MQ=255)                                                                    |                                                                         AACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGCCCTGCAAGAAGTAGTTAACCATCACCGCAGGTTCTTCAAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGC  >  REL606/2409110‑2409249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑